Abstract:In the present paper, taking Tiegunyin tea as an example, surface enhanced Raman scattering (SERS) spectrum of tea was obtained, the effects of different active substrates were explored from the aspects of enhanced factor, background noise, repeatability, and signal to noise ratio (SNR), while the impact of adsorption time on the measurement was also discussed. The results show that it is feasible to obtain the SERS spectrum of tea, and Raman signal of SERS spectrum was greatly enhanced compared with that of regular Raman spectrum. The active substrate of silver colloid prepared by the reduction of silver nitrate and trisodium citrate has a better enhanced effect, while different adsorption times have no direct influences on the SERS measurement. The method based on SERS firstly proposed in this paper may provide an alternative method for the discriminant analysis and quality identification of tea.
Key words:Surface enhance Raman scattering (SERS) spectrum;Tea;Active substrate;Adsorption time
[1] LI Xue-lei, LI Qiong-yu (李雪蕾,李琼瑜). Science Technology and Industry(科技和产业), 2010, 10(8): 7. [2] ZHAO Jie-wen, GUO Zhi-ming, CHEN Quan-sheng(赵杰文, 郭志明, 陈全胜). Journal of Jiangsu University-Natural Science Edition(江苏大学学报·自然科学版), 2010, 31(3): 249. [3] Wang L F, Lee L Y, Chung J O, et al. Food Chemistry, 2008, 109(1): 196. [4] ZHANG Jia-ling, ZHANG Wei, ZHOU Zhi-gui, et al(张佳玲,张 伟,周志贵,等). Chinese Journal of Chromatography(色谱), 2011, 29(7): 681. [5] LI Guang, ZHAO Yan-li, ZHANG Rong-xiang, et al(李 光,赵艳丽,张荣香,等). Journal of Hebei Univesity-Natural Science Edition(河北大学学报·自然科学版), 2009, 29(4): 361. [6] HAN Bing, LIN Ming-xing, DING Feng-hua(韩 冰,林明星,丁凤华). Agricultural Equipment & Vehicle Engineering(农业装备与车辆工程), 2008, 207(10): 24. [7] YUAN Lin-ying, ZHOU Zheng-ke, PI Li, et al(袁林颖,周正科,皮 利,等). South China Agriculture(南方农业), 2010,(1): 80. [8] XU Yi-ming (许以明). Raman Spectroscopy and Its Application(拉曼光谱及其在结构学中的应用). Beijing: Chemical Industry Press(北京:化学工业出版社), 2005. 2: 4. [9] CHEN Yong-jian, CHEN Rong, LI Yong-zeng, et al(陈永坚,陈 荣,李永增,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2011, 31(11): 2961. [10] Chen Y J, Xi G Q, Chen R, et al. Journal of Molecular Structure, 2011, 1006: 559. [11] XI Gang-qin, CHEN Yan-ping, CHEN Rong, et al(席刚琴,陈燕坪,陈 荣,等). Chinese Journal of Lasers(中国激光), 2011, 38(9): 0904001. [12] Dana Cialla, Anne Mrz, René Bhme, et al. Analytical and Bioanalytical Chemistry, 2012, 403(1): 27. [13] Lee P C, Meisel D. Journal of Physical Chemistry, 1982, 86(17): 3391. [14] Nicolae Leopold, Bernhard Lendl. Journal of Physical Chemistry B, 2003, 107: 5723. [15] PU Xiao-ya, YUAN Yi-jun, WANG Ting-pu, et al(蒲晓亚,袁毅君,王廷璞,等). Journal of Tianshui Normal University(天水师范学院学报), 2011, 31(2): 40. [16] Zhao J, Lui H, McLean D I, et al. Applied Spectroscopy, 2007, 61(11): 1225.