Abstract：The objective of the present paper was to study the effect of monomethoxypolyethlene glycol (mPEG) modification on the stability of chicken IgY and compare the stability of the modification products by Fourier transform infrared spectroscopy (FTIR), CD spectrooscopy and fluorescence spectroscopy. NHS-mPEG was used to modify IgY after mPEG was activated with N-hydroxysuccinimide (NHS). The optimal reaction condition for modification was 1∶10 molar rate of IgY to mPEG at pH 7, reaction for 1 h, and the product was obtained with modification rate of 20.56% and activity reservation of 87.62%. In addition, the thermal and pH stability of IgY and mPEG-IgY was compared by spectroscopic methods. The results showed that the α-helix, β-sheet, β-turn, and random content of IgY changed from 14.5%, 42.1%, 6.2% and 37.2% to 1.6%, 55.25%, 5.8% and 37.5%, while mPEG changed from 12.9%, 42.7%, 6.3% and 38.1% to 3.1%, 50.5%, 7.2% and 39.2%, respectively, after incubating for 120 min at 70 ℃. For the treatment with acid-base, similarly, the structure changes of mPEG-IgY were smaller than IgY. Thus, it is indicated that IgY modified by mPEG had greater stable properties.
王丽英，马美湖*，黄 群，史晓霞. 鸡卵黄免疫球蛋白的mPEG修饰及其稳定性研究[J]. 光谱学与光谱分析, 2012, 32(09): 2501-2507.
WANG Li-ying, MA Mei-hu*, HUANG Qun, SHI Xiao-xia. Study on the Stability of Chicken Egg Yolk Immunoglobulin (IgY) Modified with mPEG. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(09): 2501-2507.
[1] Loeken M R, Roth T F. Immunology, 1983, 49(1): 21. [2] Da Silva W D, Tambourgi D V. Veterinary Immunology and Immunopathology, 2010, 135: 173. [3] TAO Wei-sun, SUN Wei, JIANG Yong-ming(陶慰孙, 孙 惟, 姜涌明). The Foundation of Protein Molecule(蛋白质分子基础). Beijing: Higher Education Press(北京：高等教育出版社), 1995. [4] Piskarev V E, Navrátil J, Karásková H, et al. Biochemical Journal, 1990, 270: 755. [5] Veronese F M, Pasut G. Drug Discovery Today, 2005, 10(21): 1451. [6] TIAN Shuo, XU Chen, YAO Wen-bing(田 硕，徐 晨，姚文兵). China Biotechnology(中国生物工程杂志), 2010, 30(5): 122. [7] Veronese F M. Biomaterials, 2001, 22: 405. [8] Akita E M, Nakai S. Journal of Immunological Methods, 1993, 160(2): 207. [9] WANG Li-ying, MA Mei-hu, CAI Zhao-xia, et al(王丽英, 马美湖, 蔡朝霞, 等). Chinese Journal of Chromatography(色谱), 2012, 1(30): 80. [10] Sousa C M, Miranda R M, Freire R B. Brazilan Archives of Biology and Technology, 2011, 54(4): 643. [11] Weiner L, Shin I, Shimon J W L, et al. Protein Science, 2008, 18: 196. [12] Talia M, Wilchek M. Bioconjugate Chemistry, 1993, 4: 586. [13] Kanako K, Naomi T, Yoshiki S. Pharmaceutical Bulletin, 2010, 33(7): 1253. [14] LI Shan-shan, WANG Jia-qi, WEI Hong-yang, et al(李珊珊, 王加启, 魏宏阳, 等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2008，24(S2): 283. [15] Wittenboer A, Niemeijer B, Karmee S K, et al. Journal of Molecular Catalysis B: Enzymatic, 2010, 67: 208.
