Detection and Analysis of Polycyclic Aromatic Hydrocarbons Using Surface-Enhanced Raman Spectroscopy
MA Jun1, LIU Shu1, SHI Xiao-feng1,2, HAN Xiao-hong1, KONG De-di1, ZHENG Rong-er1
1. Optics & Optoelectronics Laboratory, Ocean University of China, Qingdao 266100, China 2. College of Science, Qingdao Agricultural University, Qingdao 266109, China
Abstract：In the present paper, the gold colloid with parameters optimized was used as surface-enhanced Raman scattering(SERS) active substrate to realize the trace detection of polycyclic aromatic hydrocarbons(PAHs) in water for the first time. Gold colloids with different size were prepared using chemical reduction method, and the optimum size selected at 632.8 nm excitation wavelength by experiment is (32±3) nm. The influence of pH value on the enhancement of PAHs was researched, and the optimal pH value is 13. Spectral intensity increased by approximately 20-fold compared with pH 6. The SERS spectra of naphthalene, phenanthrene and pyrene aqueous solutions were detected by the optimum gold colloid, and the minimum concentrations obtained were 20, 4 and 4nmol·L-1, respectively. There was a linear relationship between peak intensity and concentration, and the linear regression correlation coefficients were all above 0.985. For the mixture, the authors could distinguish each PAH easily for their own characteristic peaks. The experimental results show that such active substrate has a very high sensitivity as well as good application prospect.
Key words：Surface-enhanced saman spectroscopy;Polycyclic aromatic hydrocarbons;Gold colloid;pH value
马 君1，刘 澍1，史晓凤1,2，韩晓红1，孔德地1，郑荣儿1 . 多环芳烃的表面增强拉曼光谱探测与分析[J]. 光谱学与光谱分析, 2012, 32(09): 2452-2457.
MA Jun1, LIU Shu1, SHI Xiao-feng1,2, HAN Xiao-hong1, KONG De-di1, ZHENG Rong-er1 . Detection and Analysis of Polycyclic Aromatic Hydrocarbons Using Surface-Enhanced Raman Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(09): 2452-2457.
[1] ZHAO Wen-chang, CHENG Jin-ping, XIE Hai-zhui, et al(赵文昌, 程金平, 谢海赘, 等). Periodical of Ocean University of China(环境科学与技术), 2006, 29(3): 105. [2] LI Xian-guo, GUO Xin-yun, ZHOU Xiao(李先国, 虢新运, 周 晓). Periodical of Ocean University of China(中国海洋大学学报), 2008, 38(3): 473. [3] Fleischmann M, Hendra P, McQuilla A J. Chemical Physics Letters, 1974, 26(2): 163. [4] Kneipp K, Wang Y, Kneipp H, et al. Physical Review Letters, 1997, 78(9): 1667. [5] Nie S M, Emory S R. Science, 1997, 275(5303): 1102. [6] Moskovits M. Rev. Mod. Phys., 1985, 57(3): 783. [7] Otto A. Journal of Raman Spectroscopy, 2005, 36(12): 497. [8] Olson L G, Uibe R H, Harris J M, et al. Applied Spectroscopy，2004, 58(12): 1394. [9] Peron O, Rinnert E, Lehaitre M, et al. Talanta, 2009, 79(2): 199. [10] Jones C L, Bantz K C, Haynes C L, Analytical and Bioanalytical Chemistry, 2009, 394(1): 303. [11] Xie Y, Wang X, Han X, et a1. Journal of Raman Spectroscopy, 2011, 42(5): 945. [12] WANG Jian, ZHU Tao, ZHANG Xu(王 健, 朱 涛, 张 续). Chinese Journal of Chemical Physics(物理化学学报), 1999, 15(5): 476. [13] Sanchez-Cortes S, Garcia-Ramos J V. Surface Science, 2001, 473(1-2): 133. [14] Frens G. Nature Physical Science, 1973, 241(1): 20. [15] LIU Kun, WU Shi-fa(刘 琨, 吴世法). Chinese Journal of Light Scattering(光散射学报), 2006, 17(4): 332. [16] WU Jie, ZHOU Guang-ming, LIU Lan, et al(吴 捷, 周光明, 刘 兰, 等). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2008, 25(6): 1171. [17] Costa J C S, Sant’Ana A C, Corio P, et al. Talanta, 2006, 70(5): 1011.
