Characterization of Soil Humus by FTIR Spectroscopic Analyses after Being Inoculated with Different Microorganisms Plus Wheat Straw
WANG Shuai1, 2, DOU Sen1*, LIU Yan-li1,3, LI Hui-min1, CUI Jun-tao1, ZHANG Wei1, WANG Cheng-yu1
1. College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, China 2. Popularization Center of Agricultural Technology of Jilin City, Jilin 132013, China 3. Academy of Agriculture and Forestry Sciences of Cangzhou City, Cangzhou 061000, China
Abstract：The effects of different microbial communities on the structural characteristics of humus from the black soil amended with wheat straw were studied by FTIR Spectroscopy. The results indicated that (1) The structure and amount of functional groups in the water soluble substances (WSS) was tremendously influenced by the tested microorganisms, of which the amino and aryl ether was degraded rapidly in the inoculation process, and in the meantime, the content of hydroxyl groups was significantly reduced. The bacteria was helpful to increasing the amount of aliphatic hydrocarbons, while the other inoculated treatments were contrary. At the end of culture, the phenols and polysaccharides were gradually consumed, but the content of carboxyl groups had an increasing trend. (2) In the aspect of reducing hydroxyl groups of fulvic acid (FA), the role of actinomycetes was the biggest. The fungi had the biggest effect in improving the net generation of FA content. In addition, the fungi was conducive to improve the contents of carboxyl groups and carbohydrates of FA fraction. Except the mixed strains, the other treatments were all beneficial to the degradation of polysaccharide in the FA fraction, whose rate was greater than the decomposition of lipids. (3) The bacteria, actinomycetes and fungi were all helpful to reducing the amount of aliphatic hydrocarbons of HA fraction except the mixed strains. The content of carboxyl was effectively increased by fungi, but the effect of bacteria was contrary. The tested microorganisms could consume and utilize the polysaccharides of HA fraction, which could transform the humic-like fractions from plant residues into the real humus of soil.
王 帅1，2，窦 森1*，刘艳丽1，3，李慧敏1，崔俊涛1，张 伟1，王呈玉1 . 微生物对黑土添加麦秸后腐殖质结构特征影响的红外光谱研究[J]. 光谱学与光谱分析, 2012, 32(09): 2409-2413.
WANG Shuai1, 2, DOU Sen1*, LIU Yan-li1,3, LI Hui-min1, CUI Jun-tao1, ZHANG Wei1, WANG Cheng-yu1 . Characterization of Soil Humus by FTIR Spectroscopic Analyses after Being Inoculated with Different Microorganisms Plus Wheat Straw. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(09): 2409-2413.
[1] DOU Sen，LI Kai，CUI Jun-tao，et al(窦 森，李 凯，崔俊涛，等). Acta Pedologica Sinica(土壤学报)，2008，45(6)：1148. [2] HUANG Chang-yong(黄昌勇). Soil Science(土壤学). Beijing：China Agriculture Press(北京：中国农业出版社)，2000. 34. [3] Droussi Z，D’Orazio V，Hafidi M，et al. Journal of Hazardous Materials，2009，163：1289. [4] Lima D L D，Santos S M，Scherer H W，et al. Geoderma，2009，150: 38. [5] Zhang Y L，Du J Z，Zhang F F，et al. Estuarine, Coastal and Shelf Science，2011，93：220. [6] Li X W，Xing M Y，Yang J，et al. Journal of Hazardous Materials，2011，185：740. [7] He X S，Xi B D，Wei Z M，et al. Chemosphere，2011，82：541. [8] Chai X L，Shimaoka T，Cao X Y，et al. Chemosphere，2007，69：1446. [9] Abouelwafa R，Amir S，Souabi S，et al. Bioresource Technology，2008，99：6112. [10] Gondar D，Lopez R，Fiol S，et al. Geoderma，2005，126：367. [11] Filip Z，Tesaová M. International Biodeterioration & Biodegradation，2004，54：225. [12] Zhang J J，Hu F，Li H X，et al. Applied Soil Ecology，2011，51：1. [13] González-Pérez M，Torrado P V，A. Colnago L A，et al. Geoderma，2008，146：425. [14] Benites V M，Mendonca E S，Schaefer C E G R，et al. Geoderma，2005，127：104. [15] XIAO Yan-chun，DOU Sen(肖彦春，窦 森). Chinese Journal of Analytical Chemistry(分析化学)，2007，11(35)：1596.
