SERS Spectroscopy Study of Three Pathogenic Bacteria
SU Yong-bo1, 3, SI Min-zhen1, 2*, ZHANG De-qing2, LIU Ren-ming2, LIN Feng-chun3
1. Department of Physics and Electron, Yunnan Normal University, Kunming 651000, China 2. Spectrum Research Institute, Chuxiong Normal University, Chuxiong 675000, China 3. Chuxiong Medical College, Chuxiong 675005, China
Abstract:The SERS spectra of staphylococcus aureus, proteus, and Escherichia coli was obtained on colloidal Ag nanoparticles prepared by the microwave method with the portable Raman spectrometer. Staphylococcus aureus have obvious Raman vibrating peak at 725, 1 330 and 1 450 cm-1, proteus have obvious Raman vibrating peaks at 650, 725, 950, 1 325 and 1 463 cm-1, while E. coli have obvious Raman vibrating peaks at 650, 950, 1 125, 1 242, 1 320 and 1 457 cm-1. Each peak was assigned preliminarily. Not only the position of Raman vibration peaks but also the intensity of the three bacteria is obviously different, so SERS can be used for identification and distinction of E. coli, staphylococcus aureus and proteus.
苏永波1,3, 司民真1, 2*,张德清2,刘仁明2,林逢春3 . 三种致病性细菌的SERS光谱研究[J]. 光谱学与光谱分析, 2012, 32(07): 1825-1828.
SU Yong-bo1, 3, SI Min-zhen1, 2*, ZHANG De-qing2, LIU Ren-ming2, LIN Feng-chun3 . SERS Spectroscopy Study of Three Pathogenic Bacteria . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(07): 1825-1828.
[1] Adak G K,Long S M,O’brien S J. Gut, 2002, 51(6): 832. [2] MAO Xue-dan, HU Jun-feng, LIU Xiu-mei(毛雪丹,胡俊峰,刘秀梅). Chinese Journal of Food Hygiene(中国食品卫生杂志),2011, 23(2):132. [3] Kang Y P, Si M Z, Liu R M, et al. J. Raman Spectroscopy, 2009, 41:614. [4] Li J F, Huang Y F, Ding Y. Nature, 2010, 464:392. [5] Zhou X F, Fang Y, Zhang P X. Spectrochimica Acta Part A, 2007, 67: 122. [6] Zhang Y T, Zhang Z J, Sun Y H. Journal of Chromatography A, 2006, 1129: 34. [7] Le Ru E C, Blackie E, Meyer M, et al. J. Phys. Chem., 2007, 111: 37. [8] Choi S H, Wang D, Williams J R, et al. Applied Surface Science, 2007, 253: 5411. [9] Yakutik I M, Shevchenko G P, Rakhmanov S K. Colloids and Surface, 2004, 242: 175. [10] Hou X M, Fang Y. Colloid and Interface Science, 2007, 316: 19. [11] Ratna T, Richard J C Brown, Martin J T Milton. Journal of Raman Spectrum, 2007, 38: 1469. [12] Jarvis R M, Goodacre R. Analytical Chemistry, 2004, 76(1): 40. [13] Zhang X, Young M A, Lyandres O, et al. Journal of the American Chemical Society, 2005, 127(12): 4484. [14] Knauer M, Ivleva N P, Liu X, et al. Analytical Chemistry, 2010, 82(7): 2766. [15] GONG Xiao-zhong, TANG Jiao-ning, LI Jun-qin, et al(龚晓钟,汤皎宁,李均钦, 等). Chinese Journal of Applied Chemistry(应用化学), 2005, 22(12): 1291. [16] YANG Sheng-hong, ZHANG Xiao-ming, ZHANG Ting-jie, et al(杨升红,张小明,张廷杰, 等). Rare Metal Materials and Engineering(稀有金属材料和技术), 2000, 29(5): 354. [17] Raveendran P, Fu A J, Wallen S A. Green Chemistry, 2006, 8(1): 34. [18] Patel K, Kapoor S, Dave D P, et al. J. Chem. Sci., 2005, 117(4): 311. [19] GUAN Ying-xun, FANG Da-wei, CHEN Lin, et al(关英勋,房大维,陈 林, 等). Chemical Industry Times(化工时刊), 2004, 18(6): 8. [20] SI Min-zhen, FANG Yan, DONG Gang, et al(司民真,方 炎,董 刚,等). Acta Photonica Sinica(光子学报), 2008, 37(5):1034. [21] ZHANG Yan, WANG Qiang-bin, et al(张 燕,王强斌). Acta Biophysica Sinica(生物物理学报), 2010, 26(8):673. [22] Kao P, Malvadkar N A, Cetinleaya M, et al. Advanced Materials, 2008, 20(18): 3562. [23] Tobias J Moritz, Douglas S Taylor, Christopher R Polage, et al. Analytical Chemistry, 2010, 82(7): 2703. [24] B Steven Luo, Min Lin, et al. Journal of Rapid Methods & Automation in Microbiology, 2008, 16: 238. [25] Maquelin K, Kirschner C, Choo-Smith L P, et al. Microbiol. Methods, 2002, 51: 255. [26] Vohnik S, Hanson C, Tuma R, et al. Protein Sci., 1998, 7: 193.