Study on the Effect of Temperature on the Conformation of Cross-Linked Collagen by Two-Dimensional Infrared Correlation Spectroscopy
CHEN Yi-hui1, 2, TIAN Hui-lin1, LI Ji-heng1, LI Guo-ying1*
1.National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China 2.College of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China
Abstract：The Fourier transform infrared spectroscopy and two dimensional correlation analysis method were applied to study a denaturing process of uncross-linked collagen and cross-linked collagen during varying temperature. It was found that the intensity of typically characteristic absorptions of collagen decreased and its peak shifted to low frequency, The amide II central absorbance peak moved to a lower frequency by about ~10 cm-1, which indicated that the inter-chain hydrogen bonds which stabilized the triple helix conformation of collagen were disrupted during thermal denaturation, resulting in a conformational change. The intensity of auto-peak at 1 515 cm-1 was maximum, which suggested that the temperature had a big impact on amide II. In comparison with uncross-linked collagen, the intensity of cross-peaks of cross-linked collagen was weaker, which demonstrated that the effect of temperature on the structure of cross-linked collagen was smaller, and the thermal stability properties of collagen solution could be improved by cross-linking. While the order of second structure changes of cross-linked collagen was different. These fundamental data should provide available information for understanding the relationship between the structure and function of cross-linked collagen.
陈以会1, 2，田荟琳1，李季衡1，李国英1* . 二维红外相关光谱法研究温度对交联胶原结构的影响[J]. 光谱学与光谱分析, 2012, 32(06): 1500-1506.
CHEN Yi-hui1, 2, TIAN Hui-lin1, LI Ji-heng1, LI Guo-ying1* . Study on the Effect of Temperature on the Conformation of Cross-Linked Collagen by Two-Dimensional Infrared Correlation Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(06): 1500-1506.
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