Investigation of FTIR Spectra Analysis of Gallstones from Xuzhou Region by Solvent Extraction
YIN Wen-xuan1, WANG Jing1, ZHANG Nan1, WANG Jian-huai1, BAO Ya-xing2, SUN Cun-jie3, LI Zhuang-fu1
1. College of Chemical Engineering, China University of Mining Technology and Engineering, Xuzhou 221116, China 2. The First People’s Hospital of Xuzhou, Xuzhou 221000, China 3. The Second People’s Hospital of Xuzhou, Xuzhou 221000, China
Abstract：The present paper is a preliminary exploration of the possible way the gallstones are formed. Five categories of gallstones from clinical surgery in Xuzhou region were extracted by a series of solvents. Fourier transform infrared spectroscopy (FTIR) was used to characterize the structure of morphological changes between gallstone and residue by extracting. The gallstone samples are from clinical surgeries in Xuzhou region where gallstone disease is quite common. Samples were extracted with a series of solvents, and then FTIR and other instrumental analysis were applied to characterize the composition, structure and morphological changes of the samples both before and after the extraction. The results show that the gallstone samples can be classified as 5 types: cholesterol-type, cholesterol-based hybrid type with salt, bilirubin and protein as its insoluble substances, brown pigment type and black pigment type gallstones. The results also indicate that protein plays a key role in gallstone nucleation process by providing a sediment matrix for the formation of gallstones. Both cholesterol and carbonated hydroxyapatite are found in the insoluble substances of the samples. It is possible that cholesterol was accompanied by carbonated hydroxyapatite and there are interactions between them, and these interactions contribute to the crystallization process and speed up the formation of gallstones. All the results above may provide useful references for the clinical diagnosis, treatment and prevention of gallstones.
尹文萱1，王 敬1，张 楠1，王建怀1，鲍亚星2，孙存杰3，李壮福1 . 徐州地区胆结石的溶剂萃取的FTIR研究[J]. 光谱学与光谱分析, 2012, 32(04): 953-957.
YIN Wen-xuan1, WANG Jing1, ZHANG Nan1, WANG Jian-huai1, BAO Ya-xing2, SUN Cun-jie3, LI Zhuang-fu1 . Investigation of FTIR Spectra Analysis of Gallstones from Xuzhou Region by Solvent Extraction. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(04): 953-957.
[1] HUANG Zhi-qiang(黄志强). Gallstone Disease(胆石病). Beijing: Tsinghua University Press(北京: 清华大学出版社). Guangzhou: Jinan University Press(广州: 暨南大学出版社), 2002. 153. [2] OUYANG Jian-ming, ZHANG Hui-min(欧阳健明, 张慧敏). Chinese Journal of Clinical Hepatology(临床肝胆病杂志), 2006, 22(2): 156. [3] YANG Zhan-lan, WENG Shi-fu, WU Jin-guang(杨展澜, 翁诗甫, 吴瑾光). Journal of Natural Science of Peking University(北京大学学报·自然科学版), 1998, 34(4): 429. [4] Erpecum K J, Carey M C. Biochimica et Biophysica Acta, 1997, 1345: 269. [5] Miller L J, Holicky E L, Ulrich C D, et al. Gastroenterology, 1995, 109(4): 1375. [6] WU Zhi-ren, LIU Gang, QU Xin-gang, et al(邬志韧, 刘 刚, 瞿新刚, 等). Chinese Journal of Medical Physics(中国医学物理学杂志), 2008, 25(1): 503. [7] WANG Hui-zhen, WU Jun(王慧贞, 武 君). Journal of China Medical University(中国医科大学学报), 1991, 20: 10. [8] Rapacz-Kmita A, Paszkiewicz Z, et al. Molecular Structure, 2005, 744-747: 653. [9] Stewart L, Grifiss J M, Jarvis G A, et al. The American Journal of Surgery, 2006, 192: 598. [10] LIU Gang, XING Da, YANG Hai-min, et al(刘 刚, 邢 达, 杨海珉, 等). Acta Optica Sinica(光学学报), 2002, 22(4): 441. [11] Maurer K J, Carey M C, Fox J G. Gastroenterology, 2009, 136(2): 425. [12] WENG Shi-fu(翁诗甫). FTIR Spectrograph(傅里叶变换红外光谱仪). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005.
