Rapid Determination of Fatty Acids in Soybeans [Glycine max (L.) Merr.] by FT-Near-Infrared Reflectance Spectroscopy
SUN Jun-ming1,HAN Fen-xia1,YAN Shu-rong1,YANG Hua1,Tetsuo Sato2
1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China 2. National Agricultural Research Center for Kyushu Okinawa Region(KONARC), Koshi-City, Kumamoto，Japan 861-1192
Abstract：Current breeding programs dealing with fatty acid (FA) concentrations in soybean [Glycine max (L.) Merr.] require large numbers for gas chromatographic analyses, thus it is important to develop a method for rapid determination of fatty acid by Near-Infrared Reflectance spectroscopy (NIRS) in soybeans. The objective of this work was to study the potential of fourier-transform near-infrared reflectance spectroscopy (FT-NIRS) to estimate the fatty acid concentrations in Chinese soybean varieties. One hundred and eight of soybean cultivars or lines (the calibration set: 64;the external validation set: 44) were scanned within 4 000-12 500 cm-1 of wavenumbers using a standard sample cup by NIRS machinery, and analyzed the fatty acids by gas chromatograph (GC) methods. Equations were developed using partial least squares (PLS) regression and cross validation for multivariate calibration in this study. The optimal spectral region was selected from 6 101.9 to 5 446.5 cm-1 based on the OPUS 5.0 software. Cross validation results showed that major FA components such as oleic acid (R2CV=0.94), linoleic acid (R2CV=0.87), linolenic acid (R2CV=0.85), and total saturates (R2CV=0.88) were accurately determined by the proposed equations as compared with the reference data obtained by the GC method. External validation results also demonstrated that equation for oleic acid had the highest predictive ability (R2val=0.91), root mean square error of predication (RMSEP) value was 2.47 g·kg－1 dry weight, the ratios of RMSEP to the standard deviation (SD) was 0.29, which was usable for quality assurance application. Moreover, equations for palmitic acid, stearic acid, linoleic acid, linolenic acid, and total saturates were predicted with the determination coefficients ranging from 0.66 to 0.76, RMSEP values from 0.37 to 2.74 g·kg－1 dry weight, and RMSEP/SD values from 0.47 to 0.53, which could be used for sample screening. Therefore, we confirmed that a reliable estimation of the major fatty acid components is possible by using NIRS technique in soybeans.
Key words：Soybean [Glycine max (L.) Merr.];Fatty acid;FT-NIRS;Gas chromatograph
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