Progress in Combination of Gel Electrophoresis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Trace Elements Determination in Proteins
WANG Ying2, GUO Yan-li1, 2*, YUAN Hong-lin1*, WEI Yong-feng2, YAN Hong-tao2, CHEN Hui-hui2
1. State Key Laboratory of Continental Dynamics, Department of Geology, Northwest University，Xi’an 710069, China 2. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi’an 710069, China
Abstract：Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has become a very efficient and sensitive trace, ultratrace, and surface analytical technique for the in situ study of the concentration and distribution of the elements in life sciences with high spatial resolution. It is being used more and more frequently in biological, medical materials and protein research, which will lead to a better understanding of physiology and pathology process in cells and tissues. The present review mainly introduces the strategies of combination of gel electrophoresis (GE) with LA-ICP-MS for the quantification of trace elements in proteins, including the proteins separation, elements detection and calibration methods. The paper emphasizes the basic conditions of the proteins separation, focusing on the stability of proteins during GE and the treatment methods of staining and drying of the gel to enable successful detection of the elements by LA-ICP-MS. In addition, the application of GE-LA-ICP-MS in phosphoproteins, selenoproteins and metal-binding proteins is introduced in detail. The prospects and challenge for this technique are discussed as well for further study.
Key words：Laser ablation inductively coupled plasma mass spectrometry;Gel electrophoresis;Selenoproteins;Phosphoproteins;Metal-binding proteins;Trace elements
王 颖2，郭艳丽1, 2*，袁洪林1*，魏永锋2，闫宏涛2，陈慧慧2 . 凝胶电泳与激光剥蚀-电感耦合等离子体质谱联用测定蛋白质中微量元素的应用进展[J]. 光谱学与光谱分析, 2012, 32(01): 223-228.
WANG Ying2, GUO Yan-li1, 2*, YUAN Hong-lin1*, WEI Yong-feng2, YAN Hong-tao2, CHEN Hui-hui2 . Progress in Combination of Gel Electrophoresis and Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Trace Elements Determination in Proteins. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(01): 223-228.
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