Abstract:One hundred fifty Leymus chinensis samples with different growth stage, areas, and preparing method (oven-drying and shading natural dry), were selected to study the potential of determination of crude protein(CP), neutral detergent fiber(NDF)and acid detergent fiber(ADF)in the present research. The quality parameters of Leymus chinensis were firstly predicted using the near infrared reflectance spectroscopy in China. The three models were validated by cross-validation and external-validation. The results indicated that the NIRS models of Leymus chinensis quality prediction highly accessed the precision of chemical analysis. The coefficient of correlation of cross-validation of crude protein, neutral detergent fiber and acid detergent fiber were 0.963 7, 0.959 4 and 0.947 9, and the RMSECV of the three models were 1.41%, 1.27% and 1.27%, respectively; the correlation coefficients of external-validation were 0.965, 0.956 and 0.953, and all the ratios of standard deviation to root mean square error of prediction were higher than 3. Thus it can be testified that using NIRS analysis can rapidly and accurately determine the quality of Leymus chinensis. This method is of great significance for quick analysis of the trait of Leymus chinensis production and screening of breeding materials in Leymus spp. research of China.
[1] WU Bao-guo(武保国). Journal of Rural Animal-Breeding Technology(农村养殖技术), 2003, 10: 28. [2] SHI Li, DU Guang-ming, HAN Yu-jing(史 丽, 杜广明, 韩玉静). Feed Wide-Angle Reviews(饲料广角), 2010, 19: 39. [3] LI Hong,YANG Yong-fei(李 红, 杨允菲). Chinese Journal of Applied Ecology(应用生态学报), 2004, 15): 819. [4] PENG Yu-mei(彭玉梅). China Herbivores(中国食草动物), 2000, 1: 33. [5] DING Wu-rong, GAN You-min, GUO Xu-sheng,et al(丁武蓉, 干友民, 郭旭生, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2009, 29(2): 358. [6] SHI Su-jia(史苏佳). Food Research and Development (食品研究开发), 2007, 28: 177. [7] YANG Ying, FAN Ling(杨 英, 范 凌). Feed Industry(饲料工业), 2000, 21: 2. [8] BAI Qi-lin,CHEN Shao-jiang,YAN Yan-lu,et al(白琪林, 陈绍江, 严衍禄, 等). Scientia Agricultura Sinica(中国农业科学), 2006, 39: 1346. [9] ZHAO Huan-huan,HU Yue-gao,ZHAO Qi-bo(赵环环,胡跃高,赵其波). Chinese Journal of Animal Nutrition(动物营养学报), 2001, 13(4):40. [10] LI Da-qun(李大群). Optics and Precision Engineering(光学精密工程), 1989, (2): 50. [11] DING Li-min, JI Cheng,RONG Yi,et al(丁丽敏, 计 成, 戎 易, 等). China Feed(中国饲料), 1997, (1): 11. [12] Blosser T H, Reeves Iii J B, Bond J. Journal of Dairy Science, 1988, 71(2): 398. [13] Murray I. Forage Analysis by Near Infrard Reflectance Spectroscopy. Sward Management Handbook. UK; British Grassland Society,1993. 285. [14] Malley D F, Willliams P C, Stainton M P. Water Research, 1996, 30(6): 1325 [15] YAN Yan-lu,ZHAO Long-lian,HAN Dong-hai,et al(严衍禄,赵龙莲,韩东海,等). Fundamental and Application of Near Infrared Spectroscopy Analysis(近红外光谱分析基础与应用). Beijing: China Light Industry Press(北京:中国轻工业出版社),2005. 113. [16] Williams P C, Norris K H. Near Infrared Technology in the Agricultural and Food Industries. St. Paul(Minnesota, USA); American Association of Cereal Chemist,2001. 164.