Abstract:The interaction between rhein and bovine serum albumin(BSA) was studied by UV-Visible,fluorescence spectroscopy and circular dichroism in conjunction with electrochemical method. The results indicated that rhein has a powerful ability to quench the albumin’s fluorescence in a static mode. The binding constants(KA) and binding site numbers (n) obtained at different temperatures were 3.67×105, 0.99 (298 K) and 2.60×104, 0.83 (309 K) respectively. According to the thermodynamic parameters the main sorts of binding force of rhein-BSA was fixed as electrostatic. The distance between donor and acceptor in rhein-BSA was 3.28 nm based on the Frster energy transfer theory. Results of the circular dichroism and synchronous fluorescence show that the binding can cause conformation change of BSA.
梁 慧,赵 芳*,李炳奇. 光谱及电化学方法研究大黄酸与牛血清白蛋白的相互作用[J]. 光谱学与光谱分析, 2011, 31(09): 2446-2449.
LIANG Hui, ZHAO Fang*, LI Bing-qi. Studies on the Interaction of Rhein with Bovine Serum Albumin by Spectroscopic and Voltammetric Methods. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(09): 2446-2449.
[1] SUN Yan-tao, ZHANG Yu-pu, BI Shu-yun, et al(孙艳涛,张玉璞,毕淑云,等). Chemical Journal of Chinese Universities(高等学校化学学报),2009, 30(6): 1095. [2] YU Jia, WU Xiao-qing, SUN Hai-feng, et al(余 佳,吴晓晴,孙海峰,等). Pharmaceutical and Clinical Research(药学与临床研究), 2008, 16(2): 125. [3] DU Hong, SHAO Jia-qing, GU Ping, et al(杜 宏,邵加庆,顾 萍,等). China Journal of Chinese Materia Medica(中国中药杂志), 2010, 35(20): 2764. [4] Ding F, Liu W, Liu F, et al. Journal of Fluorescence, 2009, 19: 783. [5] Wang Y Q, Chen T T, Zhang H M. Spectrochimica Acta Part A, 2010, 75: 1130. [6] XIE Xian-chuan, WANG Xiao-rong, ZHANG You-kuan, et al(谢显传,王晓蓉,张幼宽,等). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(10): 1479. [7] Lakowica J R. Principles of Fluorescence Spectroscopy(Seconded). New York: Plenum Press, 1999. 237. [8] Umesha K, Seetharamappa J, Shankara S K. Spectrochimica Acta Part A, 2010, 75: 314. [9] Ding F, Zhao G Y, Huang J L, et al. European Journal of Medicinal Chemistry, 2009, 44: 4083. [10] Wu F Y, Zhang L N, Ji Z J, et al. Journal of Luminescence,2010, 130: 1280. [11] Ross P D, Subramanian S. Biochemistry, 1981, 20(11): 3096. [12] Kalyan S G, Bijaya K S, Swagata D. Chemical Physics Letters,2008, 452: 193. [13] LIU Zhe, WANG Kun-jie, DONG Yin-long, et al(刘 哲,王坤杰,董银龙, 等). Chinese Journal of Inorganic Chemistry(无机化学学报), 2010, 26(1): 72. [14] ZHANG Ya-feng, WU Ping, LIN Xin-hua(张亚峰,吴 萍,林新华). Chemical Research(化学研究), 2009, 20(1): 37.