1. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510642, China 2. Allergy and Immunology Institute, School of Medicine, Shenzhen University, Shenzhen 518060, China 3. College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, China
Abstract：The thermal denaturation of β-lactoglobulin of milk was investigated by using DSC, circular dichroism spectra, and fluorescence spectra systematically. And its corresponding immunogenicity was tested with ELISA experiments. In addition, bioinformatics methods were also used to explain the spectral characteristics and its corresponding immunogenic change, analyze the conformational changes of the protein during thermal denaturation, and discuss the relationship between conformational change of β-lactoglobulin caused by heat and immunogenic change. Results from this study should be useful to the establishment of a spectral method examining the extent of thermal denaturation of the food allergen, and be helpful to the understanding of the mechanism of immunogenic change of food allergen treated by heat.
吴序栎1，2，朱倩倩3，成小娟3，徐 宏3，刘志刚2，吴 晖1* . 牛乳β-乳球蛋白热稳定与免疫原性关系的光谱学研究 [J]. 光谱学与光谱分析, 2011, 31(08): 2205-2209.
WU Xu-li1，2, ZHU Qian-qian3，CHENG Xiao-juan3, XU Hong3, LIU Zhi-gang2, WU Hui1* . Spectroscopic Studies on the Relationship Between Heat Treatment Process and Immunogenicity of β-Lactoglobulin in Milk . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(08): 2205-2209.
[1] Wal J M. Allergy, 2001, 56: 35. [2] Mills E N, Valovirta E, Madsen C, et al. Allergy, 2004, 59(12): 1262. [3] Brownlow S, Morais J H, Cooper R, et al. Structure, 1997，(5): 481. [4] Pourpak Z, Motsafaie A, Hasan Z, et al. Immunoassay Immunochem, 2004, 25(4): 385. [5] Wal J M. Annals of Allergy, Asthma and Immnnology, 2004, 93: 2. [6] QIN Yi-de, ZOU Si-xiang(秦宜德, 邹思湘). Journal of Biology(生物学杂志), 2003, 20(2): 5. [7] Farrell H M, Jimenez F R, Bleck G T, et al. Journal of Dairy Science, 2004, (87): 1641. [8] El-Agamy E I. Small Rumi Res.，2007, 68(1): 64. [9] Vijayalakshmi L, Krishna R, Sankaranarayanan R, et al. Proteins, 2007, (71): 241. [10] Pettersen E F, Goddard T D, Huang C C, et al. Computer Chemisty, 2004, 25(13): 1605. [11] Selo I, Clement G, Bernard H, et al. Clin. Exp. Allergy, 1999, 29(8): 1055. [12] Provencher S W, Glockner J. Biochemistry, 1981, 20(1): 33. [13] Whitmore L, Wallace B A. Nucleic Acids Research, 2004, 32(2): 668. [14] JI Kun-mei, LI Meng, WU Xu-li, et al(吉坤美, 李 盟, 吴序栎, 等). Food Research and Development(食品研究与开发), 2009, 30(8): 323. [15] Kelly S M, Price N C. Current Protein and Peptide Science, 2000, 1(4): 349. [16] Hopp T P. Journal of Immunological Methods, 1986, 88: 1. [17] Hopp T P, Woods K R. Proceedings of the National Academy Science, 1982, 78(6): 3824. [18] LI Lan-hui, SUN Feng-mei, HUANG Juan, et al(李兰会, 孙丰梅, 黄 娟, 等). China Dairy Industy(中国乳球工业), 2005, 30(6): 22. [19] Dannenberg F. J. Food Chem., 1988, 53(1): 258.
