Indication of the Expression of Transgene in Rice Plant Based on Hyperspectral Remote Sensing Technique Ⅱ ——Growth Monitoring of Samples in the Contrast Experiment
LI Ru1*, CHEN Jin-song1, YUAN Ding-yang2, LIN Hui1, TAN Yan-ning2, YUE Yue-min3
1. Institute of Space and Earth Information Science, The Chinese University of Hong Kong, Hong Kong, China 2. China National Hybrid Rice R&D Center/ Hunan Hybrid Rice Research Center, Changsha 410125, China 3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
Indication of the Expression of Transgene in Rice Plant Based on Hyperspectral Remote Sensing Technique Ⅱ ——Growth Monitoring of Samples in the Contrast Experiment
LI Ru1*, CHEN Jin-song1, YUAN Ding-yang2, LIN Hui1, TAN Yan-ning2, YUE Yue-min3
1. Institute of Space and Earth Information Science, The Chinese University of Hong Kong, Hong Kong, China 2. China National Hybrid Rice R&D Center/ Hunan Hybrid Rice Research Center, Changsha 410125, China 3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China
摘要： Since the complication of monitoring and evaluating the problems about the transgenic expression and its impacts on the receptor in the transgenic crop breeding and other relevant evaluated works, the authors in the present work tried to assess the differences of spectral parameters of the transgenic rice in contrast with its parent group quantitatively and qualitatively, fulfilling the growth monitoring of the transgenic samples. The spectral parameters (spectral morphological characteristics and indices) chosen are highly related to internal or external stresses to the receipts, and thus could be applied as indicators of biophysical or biochemical processes changes of plant. By ASD portable field spectroradiometer with high-density probe, fine foliar spectra of 8 groups were obtained. By analyzing spectral angle and continuum removal, the spectral morphological differences and their locations of sample spectra were found which could be as auxiliary priori knowledge for quantitative analysis. By investigating spectral indices of the samples, the quantitative differences of spectra were revealed about foliar chlorophyll a+b and carotenoid content. In this study both the spectral differences between transgenic and parent groups and among transgenic groups were investigated. The results show that hyperspectral technique is promising and a helpful auxiliary tool in the study of monitoring the transgenic crop and other relevant researches. By this technique, quantitative and qualitative results of sample spectra could be provided as prior knowledge, as certain orientation, for laboratory professional advanced transgenic breeding study.
Abstract：Since the complication of monitoring and evaluating the problems about the transgenic expression and its impacts on the receptor in the transgenic crop breeding and other relevant evaluated works, the authors in the present work tried to assess the differences of spectral parameters of the transgenic rice in contrast with its parent group quantitatively and qualitatively, fulfilling the growth monitoring of the transgenic samples. The spectral parameters (spectral morphological characteristics and indices) chosen are highly related to internal or external stresses to the receipts, and thus could be applied as indicators of biophysical or biochemical processes changes of plant. By ASD portable field spectroradiometer with high-density probe, fine foliar spectra of 8 groups were obtained. By analyzing spectral angle and continuum removal, the spectral morphological differences and their locations of sample spectra were found which could be as auxiliary priori knowledge for quantitative analysis. By investigating spectral indices of the samples, the quantitative differences of spectra were revealed about foliar chlorophyll a+b and carotenoid content. In this study both the spectral differences between transgenic and parent groups and among transgenic groups were investigated. The results show that hyperspectral technique is promising and a helpful auxiliary tool in the study of monitoring the transgenic crop and other relevant researches. By this technique, quantitative and qualitative results of sample spectra could be provided as prior knowledge, as certain orientation, for laboratory professional advanced transgenic breeding study.
LI Ru1*, CHEN Jin-song1, YUAN Ding-yang2, LIN Hui1, TAN Yan-ning2, YUE Yue-min3. Indication of the Expression of Transgene in Rice Plant Based on Hyperspectral Remote Sensing Technique Ⅱ ——Growth Monitoring of Samples in the Contrast Experiment[J]. 光谱学与光谱分析, 2011, 31(06): 1621-1626.
LI Ru1*, CHEN Jin-song1, YUAN Ding-yang2, LIN Hui1, TAN Yan-ning2, YUE Yue-min3. Indication of the Expression of Transgene in Rice Plant Based on Hyperspectral Remote Sensing Technique Ⅱ ——Growth Monitoring of Samples in the Contrast Experiment. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(06): 1621-1626.
[1] Holst-Jensen A. Biotechnology Advances, 2009, 27: 1071. [2] Hurburgh C, Rippke G, Heithoff C, et al. Proceedings PITTCON, 2000. 12. [3] Roussel S, Hardy C, Hurburgh C, et al. Applied Spectroscopy, 2001, 55: 1425. [4] RUI Yu-kui, LUO Yun-bo, HUANG Kun-lun, et al(芮玉奎，罗云波，黄昆仑，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(10): 1581. [5] Kumar K S，Kang S H. Electrophoresis, 2007, 28: 4247. [6] Chalfie M. Proceedings of the National Academy of Sciences, 2009, 106: 10073. [7] Krn M, Elston T, Blake W，et al. Nature Reviews Genetics, 2005, 6: 451. [8] Raser J，O’Shea E. Science, 2005, 309: 2010. [9] Huang Y, Li L, Chen Y, et al. Science in China Series C: Life Sciences, 2006, 49: 519. [10] Baulcombe D. Nature, 2004, 431: 356. [11] Van Blokland R, Van der Geest N, Mol J，et al Plant Journal, 1994, 6: 861. [12] LI Ru, CHEN Jin-song, YUAN Ding-yang, et al(李 儒, 陈劲松, 袁定阳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2010, 30(1): 202. [13] Sohn Y，Rebello N. Photogrammetric Engineering and Remote Sensing, 2002, 68: 1271. [14] Lagacherie P, Baret F, Feret J, et al. Remote Sensing of Environment, 2008, 112: 825. [15] Murchie, E H, Horton P. Plant, Cell and Environment, 1997, 20(4): 438. [16] Gitelson A A, Gritz Y，Merzlyak M N. Journal of Plant Physiology, 2003, 160: 271. [17] Bannari A, Khurshid K S, Staenz K，et al. IEEE Transactions on Geoscience and Remote Sensing, 2007, 45: 3063. [18] Chappelle E W, Kim M S，Mcmurtrey J E. Remote Sensing of Environment, 1992, 39: 239. [19] Datt B. Remote Sensing of Environment, 1998, 66: 111. [20] Kim M, Daughtry C, Chappelle E, et al. In Proceedings of the ISPRS Sixth International Colloquium on Physical Measurements and Signatures in Remote Sensing, 17-21 January 1994, Val d’Isere, France (Paris: European Space Agency), 1994. 299. [21] Haboudane D, Miller J R, Tremblay N, et al. Remote Sensing of Environment, 2002, 81: 416. [22] Ustin S L, Gitelson A A, Jacquemoud S, et al. Remote Sensing of Environment, 2009, 113: S67. [23] Gamon J A, Peňuelas J, Field C B. Remote Sensing of Environment, 1992, 41: 35. [24] Gitelson A A, Zur Y, Chivkunova O B，et al. Photochemistry and Photobiology, 2002, 75: 272. [25] Broge N H，Leblanc E. Remote Sensing of Environment, 2001, 76：156. [26] Gitelson A A, Keydan G P，Merzlyak M N. Geophysical Research Letters, 2006, 33: L11402.
