Synthesis, Spectral Analysis, Antibacterial and Antitumor Activities of Co(Ⅱ) and Ni(Ⅱ) Ofloxacin Complexes
WANG Yan-lou1,2,FU Xu-chun3,LEI Qun-fang1,CHEN Wei1,WANG Guo-ping1*
1. College of Science, Zhejiang University, Hangzhou 310027, China 2. Ninth Oil Recovery Plant of Daqing Oilfields Co., Daqing 163853, China 3. Department of Pharmacy, City College, Zhejiang University, Hangzhou 310015, China
Abstract：The interactions of cobalt sulphate and nickel acetate with ofloxacin (ofo, Ⅰ), a 4-quinoline derivative, were studied. The hydrothermal technique was adopted in this work. The isolated solid complexes were characterized by elemental analysis, infrared spectra, electronic spectra, fluorescence spectra and thermal analysis. The results support the formation of complexes of the formula Co(ofo)2·4H2O (Ⅱ) and Ni(ofo)2·4H2O (Ⅲ). The infrared spectra of the isolated solid complexes suggested that ofo acts as bidentate ligands through one of the oxygen atoms of the carboxylic group and the ring carbonyl oxygen atom. Thermogravimetric (TG) and its differential (DTG) were carried out for the complexes. The data obtained indicated that the thermal decomposition of the two complexes in inert atmosphere proceeded approximately with two main degradation steps. Ⅰ showed a intense fluorescence in solid state at λex/λem=365 nm/461 nm, and two complexes displayed weakly similar emission maximum at 470 nm in the powder samples at ambient temperature, while the emission of Ⅱ and Ⅲ may be mainly originated from the intraligand excited states of Ⅰ. Ⅰ, Ⅱ and Ⅲ were assayed against two kinds of gram-positive and two kinds of gram-negative bacteria by in vitro doubling dilutions method. The results indicated that Ⅱ and Ⅲ have the similar minimal inhibitory concentration (MIC) as the Ⅰ against S. Aureus, M. Lutens, E. Coli and P. Aeruginosa. The inhibitory effect of Ⅰ, Ⅱ and Ⅲ on leukemia HL-60 cell line has been measured by using MTT (Methyl-Thiazol-Tetrozolium) method and that on liver cancer BEL-7402 cell line measured by SRB (Sulphurhodamin B) method. The results indicated that the complexes in the high concentration have inhibitory effect on HL-60 cell line.
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