Determination of Fat, Protein and DM in Raw Milk by Portable Short-Wave Near Infrared Spectrometer
LI Xiao-yun1, WANG Jia-hua2, HUANG Ya-wei1, HAN Dong-hai1*
1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China 2. College of Chemistry and Chemical Engineering, Xuchang University, Xuchang 461000, China
Abstract：Near infrared diffuse reflectance spectroscopy calibrations of fat, protein and DM in raw milk were studied with partial least-squares (PLS) regression using portable short-wave near infrared spectrometer. The results indicated that good calibrations of fat and DM were found, the correlation coefficients were all 0.98, the RMSEC were 0.187 and 0.217, RMSEP were 0.187 and 0.296, the RPDs were 5.02 and 3.20 respectively; the calibration of protein needed to be improved but can be used for practice, the correlation coefficient was 0.95, RMSEC was 0.105, RMSEP was 0.120, and RPD was 2.60. Furthermore, the measuring accuracy was improved by analyzing the correction relation of fat and DM in raw milk. This study will probably provide a new on-site method for nondestructive and rapid measurement of milk.
Key words：Portable short-wave near infrared spectrometer;Diffuse reflectance;Milk components;PLS
李晓云1，王加华2，黄亚伟1，韩东海1* . 便携式近红外仪检测牛奶中脂肪、蛋白质及干物质含量 [J]. 光谱学与光谱分析, 2011, 31(03): 665-668.
LI Xiao-yun1, WANG Jia-hua2, HUANG Ya-wei1, HAN Dong-hai1* . Determination of Fat, Protein and DM in Raw Milk by Portable Short-Wave Near Infrared Spectrometer . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(03): 665-668.
[1] Behmer D. Food Science and Technology, 2009, 23(4): 37. [2] Karoui R, De Baerdemaeker. J. Food Chemistry, 2007, 120(3): 621. [3] Feng Diwu, Chen Shuijuan, Yang Xiaojing, et al. Proceedings-1st International Congress on Image and Singal Processing, 2008, 5: 133. [4] Shao Yongni, He Yong, Feng Shuijuan. Food Research International, 2007, 40: 835. [5] Ali Sultaneh, Harald Rohm. Dairy Technology, 2007, 60(4): 241. [6] PI Fu-wei, WANG Yan-ling, LU Chao, et al(皮付伟，王燕岭，鲁 超，等). Modern Scientific Instruments(现代科学仪器), 2006, 4: 34. [7] Slobodan Sasic, Yukihiro Ozaki. Anal. Chem., 2001, 73: 64. [8] WANG Yun, XU Ke-xin, CHANG Min(王 云，徐可欣，常 敏). Optical Instruments(光学仪器), 2006, 28(3): 6. [9] Williams P C. Journal of Near Infrared Spectroscopy, 1993, 1: 25. [10] Malley D F, Findlay D L, Zippel B. Journal of Paleolimnolopy, 1999, 21(3): 295. [11] GAO Wen-ji, YUAN Hong-fu, QIU Teng, et al(高文骥，袁洪福，邱 藤，等). Chemical Journal of Chinese Universities(高等学校化学学报), 2009, 30(7): 1297. [12] TU Zhen-hua, ZHU Da-zhou, JI Bao-ping, et al(屠振华, 朱大洲, 籍保平, 等). Chinese Journal of Analytical Chemistry(分析化学), 2010, 38(1): 45. [13] XU Yun, WANG Yi-ming, WU Jing-zhu, et al(徐 云, 王一鸣, 吴静珠, 等). Journal of Agricultural Mechanization Research(农机化研究), 2010, 1: 51. [14] WANG Yun, XU Ke-xin, CHANG Min(王 云，徐可欣，常 敏). Optical Instruments(光学仪器), 2006, 28(3): 6.
