Laser Tweezers Raman Spectroscopy Analysis of Cold-Adapted Aromatic Hydrocarbons-Degradating Strains Isolated from Antarctic Sea
WANG Yi-bin1, 2, MIAO Jin-lai1, 2*, HE Bi-juan3, LIANG Qiang1, 2, LIU Fang-ming1, 2, ZHENG Zhou1, 2
1. First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China 2. Key Lab of Marine Bioactive Substances, State Oceanic Administration, Qingdao 266061, China 3. Guangxi Academy of Sciences, Nanning 530007, China
Abstract：Laser tweezers Raman spectroscopy can help with observing and studying individual cells or organelles in a natural state for a relatively long period. In the present experiment, Laser tweezers Raman spectroscopy (LTRS) was used as a tool to report physiological metabolism such as cells growth and nucleic acid, proteins, lipid and glucose of a single active cold-adapted Aromatic hydrocarbons-degradating strains isolated from Antarctic Sea. After the Raman spectrum was collected and analyzed, the findings are as follows: Raman spectrum identified the components of a single cold-adapted Aromatic hydrocarbons-degradating strain and there were more proteins and carbohydrate produced during the Planococcus sp.NJ41 and Shewanella sp.NJ49 growth and degradation; but there was more lipid than the proteins produced during the Pseudoalteromonas sp.NJ289 growth and degradation; the amount of proteins produced by the strains corresponds with the production of degradation rate-limiting enzyme, and was also related to the capacity of low-temperature degradation of aromatic hydrocarbons.
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