In Vivo Reflectance Spectroscopy Study of Different Clearing Agents on Human Skin Optical Clearing
ZHAO Qing-liang1, GUO Zhou-yi1, WEI Hua-jiang1*, YANG Hong-qin2, SI Jun-li3, XIE Shu-sen2, WU Guo-yong4, ZHONG Hui-qing1, LI Lan-quan1, GUO Xiao1
1. Key Laboratory of Laser Life Science, Ministry of Education, Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China 2. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China 3. The Second Affiliated Clinical Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China 4. Department of Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
Abstract：The changes of skin tissue reflectance spectroscopy before and after being treated with the optical clearing agents of three different types of optical clearing within the wavelength rang of 400～1 000 nm, and the degree of changes in reflectance spectroscopy of each group skin during 0～60 min at 580 nm in vivo were real-time dynamically researched. The reflectance spectroscopy of skin tissue before and after being dealt by the optical clearing agents of glycerol, glucose and propylene glycol was measured using a USB-4000 fiber spectrophotometer at 0, 10, 20, 30, 40, 50 and 60 min. The results showed that the reflectance spectral intensity was distinctly decreased, but the reflectance was significantly increased gradually with the time prolonged. However, different optical clearing agents have different clearing progress. The relative decrease of reflectance of palm skin tissue before and after being dealt by the optical agents of 40% glycerol, 40% glucose and 40% propylene glycol during 10, 20, 30, 40, 50 and 60 min at the wavelength 580 nm is 5%, 7%, 9%, 10%, 11% and 12%, 9%, 13%, 16%, 19%, 21% and 22%, and 14%, 22%, 29%, 32%, 34% and 35%, respectively. The significant improvement in light transmittance and enhancement of light penetration through tissue was demonstrated for all solutions. The effect and processes of optical clearing of skin tissue is not only closely related to the choosing of the clearing agent type, but also related to the treatment time with the skin tissue. The clearing progress of different type optical clearing agent showed the order of 40% propylene glycol, 40% glucose and 40% glycerol.
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