Abstract：The effects of ultraviolet-B (UV-B) radiation on protein structure in photosystem Ⅱ (PSⅡ) was studied using Fourier transform infrared spectroscopy. The results demonstrated that irradiation of the plant with enhanced UV-B caused the change in the structures of protein in PSII. The intensity of α-helix, β-sheet was increased, while the intensity of β-turn was decreased. The coupling of those structures with surrounding became weaker with UV-B radiation. Otherwise, the UV-B influenced the structure of tyrosine residue and made the polarity of tyrosine decrease. The changes caused by UV-B radiation could alter the combination between Mn cluster and pigments with protein inevitably. It would lead to a series of changes, such as the process of light transmission and conversion, electron transfer and oxygen evolution.
[1] Bischof K, Peralta G, Krbs G, et al. Journal of Experimental Botany, 2002, 53(379): 2411. [2] Kakani V G, Reddy K R, Zhao D, et al. Annals of Botany, 2003, 91(7): 817. [3] Barbato R, Bergo E, Szabò I, et al. The Journal of Biological Chemistry, 2000, 275(15): 10976. [4] Bergo E, Segalla A, Giacometti G M, et al. Journal of Experimental Botany, 2003, 54(388): 1665. [5] Szilárd A, Sass L, Deák Z, et al. Biochimica et Biophysica Acta, 2007, 1767(6): 876. [6] Prabha G L, Kulandaivelu G. Plant Science, 2002, 162 (5)： 663. [7] Bergantino E, Segalla A, Brunetta A, et al. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(25): 15265. [8] LIN Fen-fang, CHEN Zhu-lu, WANG Ke, et al(林芬芳, 陈祝炉, 王 珂, 等). Journal of Infrared Millimeter Waves(红外与毫米波学报), 2009, 28(4): 277. [9] DU Lin-fang(杜林方). Plant Physiology Communication(植物生理学通讯), 1995, 31(3): 212. [10] De Las Rivas J, Barber J. Biochemical Journal, 1997, 36(29): 8897. [11] LU Wei, XU Chun-he, LI Rong, et al(陆 卫，徐春和，李 荣，等). Journal of Infrared Millimeter Waves(红外与毫米波学报), 1998, 17(1): 66. [12] Ruan X，Wei J，Xu Q，et al. Journal of Molecular Structure，2000，525(1)： 97. [13] Ségui J A, Maire V, Gabashvili I S, et al. Journal of Photochemistry and Photobiology B: Biology, 2000, 56(1): 39. [14] XIE Meng-xia, LIU Yuan(谢孟峡, 刘 媛). Chemical Journal of Chinese Universities(高等学校化学学报), 2003, 24(2): 226. [15] YANG Z L，LI L B，XU Y N，et al. Science in China Series C: Life Sciences, 2000, 43(3): 330. [16] Vass I, Sass L, Spetea C, et al. Biochemical Journal, 1996, 35(27): 8964. [17] Rodrigues G C, Jansen M A K, van den Noort M E，et al. Plant Science，2006, 170(2)： 283. [18] BAO Han, ZHANG Chun-xi, REN Ya-nan, et al(鲍 涵, 张纯喜, 任亚男, 等). Chinese Science Bulletin(科学通报)，2010，55(1)： 26. [19] CAO Dong, SHI Su-jia, ZHANG Yong-gang, et al(曹 栋，史苏佳，张永刚，等). Chemistry Bulletin(化学通报)，2008，(11)： 877.
