The Influence of the Redox Conditions on the Three-Dimensional Excitation-Emission Matrix (3DEEM) Fluorescence Spectroscopy of the Dissolved Organic Matter (DOM) in the Overlying Water
ZHU Wei-huang, HUANG Ting-lin*, ZHANG Ya-ning
Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi’an University of Architecture and Technology, Xi’an 710055, China
Abstract：In the present study three-dimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy was applied to characterize the dissolved organic matter (DOM) in the overlying water under aerobic and anaerobic condition. The effects of redox condition were significant on 3DEEM fluorescence spectra of DOM, and in the aerobic condition, the peak intensities of protein-like fluorophores were both higher than those of the humic-like fluorophores, however, the phenomenon of the oxidative degradation of humic-original DOM could be seen. While in anaerobic condition, the peak intensities of the humic-like fluorescence were increased with increasing the incubation time. After the 21 day anaerobic incubation, the peak intensities of the humic-like fluorescence can be as 3.51 and 3.78 times higher than those of protein-like fluorescence. The differences in the DOM fluorescence parameters, e.g., peak intensities, locations and fluorescence index, indicate the difference in the chemical structures and various origins of the DOM in the overlying water between sediment-water interfaces.
朱维晃，黄廷林*，张亚宁 . 氧化还原条件变化对上覆水体中溶解有机质的三维荧光光谱特征影响 [J]. 光谱学与光谱分析, 2010, 30(12): 3272-3276.
ZHU Wei-huang, HUANG Ting-lin*, ZHANG Ya-ning . The Influence of the Redox Conditions on the Three-Dimensional Excitation-Emission Matrix (3DEEM) Fluorescence Spectroscopy of the Dissolved Organic Matter (DOM) in the Overlying Water . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(12): 3272-3276.
[1] Coble P G. Marine Chemistry, 1996, 51(4): 325. [2] HAO Rui-xia, CAO Ke-xin，DENG Yi-wen(郝瑞霞, 曹可心, 邓亦文). Chinese Journal of Analysis Laboratory(分析试验室), 2007, 26: 41. [3] OUYANG Er-ming, ZHANG Xi-hui, WANG Wei(欧阳二明, 张锡辉, 王 伟). Water Resources Protection(水资源保护), 2007, 23: 56. [4] WANG Zhi-gang, LIU Wen-qing, LI Hong-bin(王志刚, 刘文清, 李宏斌). Acta Scientiae Circumstantiae(环境科学学报), 2006, (26): 275. [5] Wang Z, Wu Z, Tang S. Water Research, 2009, 43(6): 1533. [6] Sheng G P, Yu H Q. Water Research, 2006, 40(6): 1233. [7] Wu F, Evans R, Dillon P. Environ. Sci. Technol., 2003, 37(16): 3687. [8] FU Ping-qing, LIU Cong-qiang, WU Feng-chang(傅平青, 刘丛强, 吴丰昌). Quaternary Sciences(第四纪研究), 2004, 24(6): 695. [9] Cowie G L, Hedges J I. Organic Geochemistry, 1992, 19(1-3): 229. [10] Claus H, Filip Z. Acta Hydrochimica et Hydrobiologica, 1998, 26(3): 180. [11] Kelton N, Molot L A, Dillon P J. Water Research, 2007, 41(3): 638. [12] McKnight D, Boyer E, Westerhoff P, et al. Limnology and Oceanography, 2001, 46: 38. [13] Godshalk G L, Wetzel R G. Aquatic Botany, 1978, 5: 281. [14] Chen W, Westerhoff P, Leenheer J, et al. Environmental Science & Technology, 2003, 37(24): 5701. [15] Kristensen E, Ahmed S, Devol A. Limnology and Oceanography, 1995, 40(8): 1430. [16] Hertkorn N, Claus H, Schmitt-Kopplin P, et al. Environmental Science & Technology, 2002, 36(20): 4334. [17] Swietlik J, Sikorska E. Water Research, 2004, 38(17): 3791. [18] Cowie G L, Hedges J I. Chemical Geology, 1993, 107(3-4): 447.
