Abstract：A green house experiment was conducted to research the characteristics of tomato canopy spectral reflectance and leaf spectral reflectance under different nutrition treatments, and the relationships between spectral reflectance and the water content, chlorophyll content, as well as nitrogen content were analyzed. Substrate cultivation method was used to grow the plants. The substrate was made from a mixture of peat and vermiculite. Test area was prepared for four levels of nutrition to form nutritional stress. There were 12 seedlings under each nutritional condition and a total of 48 seedlings were planted for the experiment. The canopy reflectance and leaf reflectance were measured by an ASD handheld spectroradiometer and a FT-NIR spectrometer respectively. It was observed that the trend of tomato canopy reflectance was similar to each others. There was a reflection peak at about 550 nm, and the reflectance in the visible light region was lower than that in near-infrared region. The results of analysis also indicated that under different nutrient conditions, canopy spectral reflectance characteristics of tomato took on disciplinary change. At near-infrared bands, the reflectance gradually increased with adding nutrition, while reduced at visible light bands. The leaf spectral reflectance characteristics at near-infrared bands had the similar change with the canopy reflectance. There were four sensitive wavelengths of water at near-infrared bands: about 980, 1 450, 1 930, and 2 210 nm, and the results of single linear regression (SLR) and multi-linear regression (MLR) indicated that the reflectance at these sensitive wavelengths could be used to estimate the water content in tomato leaves. R2 were 0.590 3 and 0.743 7 respectively. NDCI as one of the most important spectral parameter was calculated by the spectral reflectance of 530 and 760 nm, and the result indicated that there existed a good correlation between NDCI and the nitrogen content, with R2=0.751 1. Meanwhile, red edge inflection points were analyzed under four nutrition treatments based on the first derivative of canopy spectral reflectance. The analysis results illustrated that red edge inflection position moved to direction of red light (long wavelength) with the nutrition supply.
Key words：Tomato;Spectral reflectance;Chlorophyll detection;Kjeldahl nitrogen;Green house
赵瑞娇，李民赞*，杨 策，杨 玮，孙 红 . 温室番茄冠层和叶片光谱特征分析及营养诊断[J]. 光谱学与光谱分析, 2010, 30(11): 3103-3106.
ZHAO Rui-jiao, LI Min-zan*, YANG Ce, YANG Wei, SUN Hong . The Canopy and Leaf Spectral Characteristics and Nutrition Diagnosis of Tomato in Greenhouse . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(11): 3103-3106.
[1] Al-Abbas A H, Barr R, Hall J D, et al. Agronomy Journal, 1974, 66: 16. [2] Hinzman L D, Bauer M E, Daughtry C S T. Remote Sensing of Environment, 1986, 19: 47. [3] Chen M, Glaz B, Gilert R A, et al. Agronomy Journal, 2002, 94: 1324. [4] TAN Chang-wei, GUO Wen-shan, ZHU Xin-kai, et al(谭昌伟，郭文善，朱新开，等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2008, 24(9): 131. [5] Thomas J R, Oerther G F. Agronomy Journal, 1972, 64: 11. [6] Shibayama M, Akiyama T A. Remote Sensing of Environment, 1993, 45: 117. [7] Horler D N, Barber L P, Ferns D C. Advanced Space Research, 1983, 25(3): 175. [8] YAO Xia, ZHU Yan, FENG Wei, et al(姚 霞, 朱 艳, 冯 伟, 等). Spectroscopy ane Spectral Analysis(光谱学与光谱分析), 2009, 29(8): 2191. [9] LI Ying-xue, ZHU Yan, TIAN Yong-chao, et al(李映雪, 朱 艳, 田永超, 等). Acta Agronomica Sinica(作物学报), 2006, 32(3): 358. [10] TANG Yan-lin, WANG Xiu-zhen, HUANG Jing-feng, et al(唐延林, 王秀珍, 黄敬峰, 等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2003, 19(1): 145. [11] YAO Lei, YANG A-ming(姚 磊, 杨阿明). Acta Agriculturae Boreali-Sinica(华北农学报)，1997, 12(2): 102. [12] WANG Cheng, ZHAO Chun-jiang, QIAO Xiao-jun, et al(王 成, 赵春江, 乔晓军，等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2008, 24(Supp2): 343. [13] LI Min-zan, HAN Dong-hai, WANG Xiu(李民赞, 韩东海, 王 秀). Spectral Analysis and Application(光谱分析技术及其应用). Beijing: Science Press(北京: 科学出版社)，2006. 8.
