The Interaction of Berberine and Human Serum Albumin
ZHAO Chang-chun1, ZHENG Wei-fa2, LI Meng-qiu2
1. Department of Chemistry, Xuzhou Normal University, Xuzhou 221009，China 2. Key Lab of Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou 221009, China
Abstract：The interaction of berberine and Human Serum Albumin(HSA) has been studied by fluorescence and UV-Vis absorption spectroscopy. With fluorescence quenching method, the binding constant K was found to be 1.168×105 L·mol-1 and the number of binding site n was 5.26. The binding distance(R=3.44 nm) and energy transfer efficiency (E=0.303) were also obtained according to Frster’s non-radiation energy transfer theory. The energy transfer path between tyrosine residual and tryptophan residual was cut by berberine in the site of HSA and weak fluorescence berberine-HSA complex was also observed.
赵长春1，郑维发2，李梦秋2 . 小檗碱与人血清白蛋白的相互作用 [J]. 光谱学与光谱分析, 2004, 24(01): 111-113.
ZHAO Chang-chun1, ZHENG Wei-fa2, LI Meng-qiu2 . The Interaction of Berberine and Human Serum Albumin . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 111-113.
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