1. Department of Chemistry, Tongji University, Shanghai 200092, China 2. School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
Abstract：The adsorption state and the characteristics of L-aspartic acid adsorbed on silver sol were studied by the Surface-Enhanced Raman Scattering (SERS) method. Strong Raman signals were detected in the experiments. The results suggested that L-aspartic acid adsorbed on the silver surfaces through carboxyl and nitrogen atoms since the signals were mainly due to the carboxyl and the nitrogen of the molecule of L-aspartic acid. The adsorption of carboxyl on the silver surfaces is chemical adsorption, which is based on the mechanism of charge-transfer, while the adsorption of nitrogen on the silver surfaces is physical type, which is due to the electromagnetic mechanism. The intensity of surface-enhanced Raman scattering of L-aspartic acid adsorbed on silver surface was also analyzed, and it was found that the intensity of surface-enhance Raman scattering will change with different concentrations of L-aspartic acid adsorbed on the silver surfaces. The intensity will reach the top value when the concentration of L-aspartic acid was 10-3 mol·L-1. When the concentration of L-aspartic acid decreased to 10-4 mol·L-1, the intensity of surface-enhanced Raman scattering became a little weaker than that with 10-3 mol·L-1. With further decrease in the concentration of L-aspartic acid, the intensity of surface-enhanced Raman scattering also decreased gradually. When the concentration of L-aspartic acid decreased to 10-6 mol·L-1, the intensity of surface-enhanced Raman scattering was very low. The above study will be helpful to the further study of peptide and other complex biological systems.
朱志良1，郜俊影1，李风亭2，张冰如2 . L-天冬氨酸在银胶体中吸附状态的表面增强拉曼光谱研究 [J]. 光谱学与光谱分析, 2004, 24(01): 68-70.
ZHU Zhi-liang1，GAO Jun-ying1，LI Feng-ting2，ZHANG Bing-ru2 . The Study of Adsorption of L-aspartic Acid on Silver Sol by Surface-enhanced Raman Scattering . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 68-70.
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