1. Department of Food Science, South China Agriculture University, Guangzhou 510642, China 2. Botany Institute of Guangxi,Guilin 541006,China 3. Sun Yat-sen Medical College,Sun Yat-sen University,Guangzhou 510089,China
Abstract:In this paper, spectral identification of methamidophos artificial antigen was reported. The results showed that UV absorbance spectrum of artificial antigens of BSAM changed compared with carrier BSA and hapten Met. The absorbance peaks which are similar to P—O—C and PO moiety in IR spectrum of Met molecule appeared in the IR spectrum of the artificial antigens of BSAM. The chemical shift of the artificial antigens of BSAM is the same as that of standard Met in 31P NMR spectrum. Phosphor concentration of the artificial antigens of BSAM was determined by phosphor molybdate blue spectrophotometry, and then conjugating ratio was calculated, to be 11. The results obtained with the above four spectrum methods showed that the artificial antigens of BSAM was synthesized successfully.
[1] LEI Jin-hai(雷进海). Contem. Chem. Indu.(现代化工),1994, 11: 22. [2] Ogawa T, Okamoto N, Taniguchi K et al. J Food Hygiene Soc. of Japan, 1997, 38: 204. [3] Tsuchiya T, Maeda K, Sekiguchi Y et al. Bull. Nation Insti. of Health Sci., 1998, 116: 157. [4] Sherry J P. Crit. Rev. Anal. Chem.,1992, 23: 217. [5] Hennion M C, Damia B. Analytica Chimica Acta,1998, 362: 3. [6] Kalidindi H, Namburi B K V, Beedu S R. J. AOAC Int.,2001, 84: 1001. [7] Kamps H C, Carlin R J, Sheffield C et al. J Immunol. Methods,1993, 164: 245. [8] Chen P S, Tonbara T Y, Wamer H. Anal. Chem.,1956, 28: 1756. [9] CHEN Xin-jian(陈新建). Application of Immunological Technic in Plant Science (免疫学技术在植物科学中的应用). Beijing:China Agriculture University Press,(北京: 中国农业大学出版社), 1998. 30. [10] Shenyang Research Institute of Chemical Industry(沈阳化工研究院). Pesticides(农药), 1973, 2: 1. [11] Thomas L C. Interpretation of the IR Spectral of Organic Phosphorus Compounds. New York: Chapman and Hall.,1982. 33. [12] Schneider P, Hammock B D. J. Agri. Food Chem.,1992, 40: 525.