Abstract:Cytoskeleton plays an important role in plant cells and their movements, developments, and so on. With MS culture medium, the present paper cultured GFP-fused Arabidopsis thaliana in asepsis condition, which completed its whole lifecycle in the lab, starting from germination, going through plant growth, blossom, and ending at fructification. During this process, the authors used two-photon laser scanning microscopy (TPLSM) which was suitable for 4D observation on large, thick, live samples to observe cytoskeleton in seed, root tip, vessel and root hair of the Arabidopsis thaliana. Dynamic root tip growth as well as the growing speed was also observed. Applying IAA to the Arabidopsis thaliana, the authors found that the growing speed of root tip was enhanced by about 3.8 times.
王鹏飞,林丹樱,马万云*. 双光子成像观测拟南芥细胞骨架及根尖生长过程[J]. 光谱学与光谱分析, 2010, 30(10): 2593-2596.
WANG Peng-fei, LIN Dan-ying, MA Wan-yun*. Observation on Cytoskeleton and Root Tip Growth of Arabidopsis Thaliana by Two-Photon Laser Scanning Microscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(10): 2593-2596.
[1] CAO Yi-zhi(曹仪植). Arabidopsis thaliana(拟南芥). Beijing: Higher Education Press(北京:高等教育出版社),2004. [2] Yoneda A, Kutsuna N, Higaki T, et al. Protoplasma, 2007, 230: 129. [3] Era A, Tominaga M, Ebine K, et al. Plant and Cell Physiology, 2009, 50(6): 1041. [4] Sheahan M B. Plant Physiology, 2004, 136: 3968. [5] Masters B R, So P T C/ Microscopy Research and Technique, 2004, 63(1): 3. [6] Potter S M. Current Biology, 1996, 6(12): 1595. [7] Nakamura O. Microscopy Research and Technique, 1999, 47: 165. [8] WANG Peng-fei, FU Jian-hua, MA Wan-yun, et al(王鹏飞,傅剑华,马万云,等). Science in China Series C: Life Sciences(中国科学 C辑:生命科学),2009,39(6):579.