Abstract：The present study was to detect and compare the content of bile acids in ox bile powder and goat gall powder of Mongolia medicine by UV. Cholic acid with sulphuric acid were heated and dehydrated,and they produced conjugated double bond. The conjugated bond showed the same absorption peak in the ultraviolet range. The method of ultraviolet spectrophotometry can be used to detect and compare the content of bile acids in ox bile powder and goat gall powder. The result showed that the linear range was 0.003 3-0.016 7 mg·mL-1 (r=0.999 7). The average recovery(n=5) of standard addition method of ox bile powder and goat gall powder was 98.48% (RSD=1.79%) and 96.46% (RSD=2.50%) respectively. The result of determination of five different samples showed that the content of bile acids in ox bile powder and goat gall powder was 40.85%-43.03% and 30.88%-32.64% respectively. The RSD of the analysis of ox bile powder and goat ball powder was 2.40% and 2.92% respectively,the RSD of stationary test of ox bile powder and goat ball powder in eight hours was 0.55% and 0.59% respectively,and the RSD of reproducibility of the analysis of ox bile powder and goat ball powder was 2.11% and 2.68% respectively. The method was simple，accurate,fast and easy to generalize and apply in many fields. It can be used to control the quality of ox bile powder and goat gall powder.
Key words：Ox bile powder;Goat gall powder;Bile acids;Ultraviolet spectrophotometry
鞠爱华,荀雅书,苏秀兰,白万富,张慧文,周秀娟. 蒙药牛胆粉与羊胆粉中胆酸类成分的分析测定[J]. 光谱学与光谱分析, 2008, 28(03): 645-647.
JU Ai-hua,XUN Ya-shu,SU Xiu-lan,BAI Wan-fu,ZHANG Hui-wen,ZHOU Xiu-juan. The Analysis of the Content of Bile Acids in Ox-Bile Powder and Goat Gall Powder of Mongolian Medicine. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(03): 645-647.
