Research on Total Arsenic Absorption of Tetrahymena Pyriformis Exposed to Arsanilic Acid Feed Additive and Its Environmental Degradation Products by HG-AFS
HAN Jing-jing1, XU Fang1*, SUN Qun2, BIAN Jing1, LI Ling-hui3
1. School of Environment Science & Engineering, Shanghai Jiaotong University, Shanghai 200240, China 2. School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China 3. Shanghai Spectrum Instruments Co., Ltd., Shanghai 200233, China
Abstract：In the present paper, a model based on the arsenic absorption of the unicellular microorganism tetrahymena pyriformis from the incubation aqueous solution of feed additive arsanilic acid and its degradation products As(Ⅲ) and As(Ⅴ) was established for the investigation of the environmental impacts of feed additive arsanilic acid on the living organisms. The tetrahymena pyriformis was incubated in the incubation aqueous solution and its degradation products for 72 h, after cleaning to remove the incubation aqueous solution, the cell was broken up and separated. The cell membrane and cytoplasm were digested with nitric acid and hydrochloric acid separately. A method of arsine generation atomic fluorescence spectrometry was used for the determination of the absorbed arsenic. Experimental findings illustrate that arsenilic acid and its degradation products As(Ⅲ) and As(Ⅴ) can be absorbed by the cell membrane then enter into cytoplasm. In addition, the degradation products As(Ⅲ) and As(Ⅴ) would more easily be absorbed than the arsanilic acid. The living organisms would be poisoned via the As absorption of the cells.
Key words：Arsanilic acid;Feed additive;Environmental release and degradation;Arsine generation atomic fluorescence spectrometry;Tetrahymena pyriformis;Arsenic poisoning of living organism
韩晶晶1，徐 芳1*，孙 群2，边 静1，李玲辉1 . 氢化物发生-原子荧光光谱法研究嗜热四膜虫对阿散酸饲料添加剂及其环境降解物的砷吸收 [J]. 光谱学与光谱分析, 2010, 30(09): 2567-2570.
HAN Jing-jing1, XU Fang1*, SUN Qun2, BIAN Jing1, LI Ling-hui3 . Research on Total Arsenic Absorption of Tetrahymena Pyriformis Exposed to Arsanilic Acid Feed Additive and Its Environmental Degradation Products by HG-AFS . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(09): 2567-2570.
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