1. 深圳大学化学与化工学院，广东 深圳 518060 2. 深圳大学医学院过敏反应与免疫学研究所，广东 深圳 518060 3. School of Biological Science, The University of Auckland, 92019, Auckland, New Zealand/NCIECP, Auckland University of Technology, Auckland, New Zealand
Spectroscopic Studies on Refolding Process in Vitro of Pan-Allergen Profilin in Coco Pollen
LUO Hai-mei1, XIAO Jie1, WU Yu-lan2, LIU Zhi-gang2, Jun Lu3, XU Hong1*
1. College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen 518060, China 2. Allergy and Immunology Institute, School of Medicine, Shenzhen University, Shenzhen 518060, China 3. School of Biological Science, The University of Auckland, 92019, Auckland, New Zealand/NCIECP, Auckland University of Technology, Auckland, New Zealand
Abstract：Recombinant proteins expressed by prokaryotic expression system are normally in the form of inclusion. In the present paper, refolding process of recombinant pan-allergen profilin protein induced by urea has been investigated by using circular dichroism spectra, fluorescence spectra, synchronous fluorescence spectra systematically. And the spectral characteristics of the renaturation were obtained. In addition, bioinformatics methods including predications of secondary and tertiary structures have also been used to explain the spectral characteristics and analyze the conformational changes of the protein during renaturation in vitro. Results from this study should be useful to the establishment of a spectral method examining the extent of protein renaturation, and be helpful to the understanding of the mechanism of renaturation of recombinant protein.
Key words：Cocos nucifera pollen;Allergen;Profilin;Refolding in vitro;Dialysis;Bioinformatics
罗海梅1，肖 杰1，邬玉兰2，刘志刚2，Jun Lu3，徐 宏1* . 椰子花粉过敏原profilin蛋白体外重折叠过程的光谱学研究[J]. 光谱学与光谱分析, 2010, 30(09): 2428-2432.
LUO Hai-mei1, XIAO Jie1, WU Yu-lan2, LIU Zhi-gang2, Jun Lu3, XU Hong1* . Spectroscopic Studies on Refolding Process in Vitro of Pan-Allergen Profilin in Coco Pollen . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(09): 2428-2432.
