Optical Properties of Human Normal Small Intestine Tissue with Theoretical Model of Optics about Biological Tissues at Ar+ Laser and 532 nm Laser and Their Linearly Polarized Laser Irradiation in Vitro
1. Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China 2. First Affiliated Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China
Abstract：A double-integrating-spheres system, basic principle of measuring technology of ray radiation, and optical model of biological tissues were used for the study. Optical properties of human normal small intestine tissue at 476.5, 488, 496.5, 514.5 and 532 nm laser and their linearly polarized laser irradiation were studied. The results of measurement showed that the total attenuation coefficient and scattering coefficient of the tissue at these wavelengths of laser and their linearly polarized laser irradiation increased with decreasing wavelengths. And obviously there was a distinction at 514.5 to 532 nm wavelength between lasers and their linearly polarized laser irradiation. Absorption coefficient of tissue at these wavelengths of laser and their linearly polarized laser irradiation increased with decreasing wavelengths. Absorption coefficient of tissue at 514.5 to 532 nm wavelength of laser was obviously decreasing，which was independent of these wavelengths of laser or their linearly polarized laser irradiation. Mean cosine of scattering of tissue at these wavelengths of laser and their linearly polarized laser irradiation also increased with decreasing wavelengths. But penetration depth of tissue at these wavelengths of laser and their linearly polarized laser irradiation also increased with increasing of wavelengths. Refractive index of tissue between these wavelengths of laser was within 1.38 to 1.48. Absorption coefficient, scattering coefficient, total attenuation coefficient, effective attenuation coefficients of tissue in Kubelka-Munk two-flux model at the same wavelength of laser and their linearly polarized laser irradiation showed no prominent distinction (P＞0.01). Absorption coefficient, scattering coefficient, total attenuation coefficient, effective attenuation coefficients of tissue in Kubelka-Munk two-flux model at different wavelength of laser and their linearly polarized laser irradiation showed obvious distinction. Optical properties of tissue at 514.5 to 532 nm wavelength of laser eｘhibited obvious distinction.
Key words：Human normal small intestine;Optical properties;Ar+ laser and 532 nm laser;Double-integrating-sphere system;Linearly polarized laser
魏华江1，邢 达1*，巫国勇2，金 鹰1，谷怀民1 . Ar+激光和532 nm激光及其线偏振激光辐照光学模型下人正常小肠组织光学特性 [J]. 光谱学与光谱分析, 2004, 24(05): 524-528.
WEI Hua-jiang1，XING Da1*，WU Guo-yong2，JIN Ying1，GU Huai-min1 . Optical Properties of Human Normal Small Intestine Tissue with Theoretical Model of Optics about Biological Tissues at Ar+ Laser and 532 nm Laser and Their Linearly Polarized Laser Irradiation in Vitro . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(05): 524-528.
[1] Vanitha Sankaran, Matthew J. Everett, Duncan J. Maitland et al. Optics Letters, 1999, 24(15): 1044. [2] Ghosh N, Mohanty S K, Majumder S K. Appl. Opt., 2001, 40(1): 176. [3] Qu Jianan, Calum MacAulay, Stephen Lam et al. Appl. Opt., 1994, 33(31): 7397. [4] Wei Huajiang, Li Xiaoyuan, Wu Guoyong et al. Chinese J. Laser, 2001, 28(6): 573. [5] Arnold D. Kim, Akira Ishimaru. Appl. Opt., 1998, 37(22): 5313. [6] Akira Ishimaru. Appl. Opt., 1989, 28(12): 2210. [7] Alfred Vogel, Christian Dlugos, Roland Nuffer et al. Lasers Surg. Med.，1991, 11: 331. [8] Graaff R, Aarnoudse J G, de Mul F F M. Appl. Opt., 1989, 28(12): 2273. [9] Seiyama A, Chen S-S, Kosaka H et al. Journal of Microscopy, 1994, 175(1): 84. [10] Cheong Wai-fung, Scott A. Prahl, Ashley J Welch. Tissues. IEEE J. Quantum. Electron., 1990, 26(12): 2166. [11] Bolin F P, Preuss L E et al. Appl. Opt., 1989, 28(12): 2297. [12] Li H, Lu Z. Proc. SPIE, 1998, 3548: 119. [13] Van der Putten W J M, Van Gemert M J C. Phys. Med. Biol., 1983, 28(6): 639. [14] Stephen T. Flock, Brian C. Wilson, Michael S Patterson. Med. Phys.，1987, 14(5): 835.
