Azoic Color Reaction of Flavonoids and Their Analytical Application
YU Qian1, CHEN Xiao-kang2, LUO Zong-ming1, YANG Chi1, MAI Hua-kun1
1. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090，China 2. Department of Chemistrｙ，Shaoguan College, Shaoguan 512005，China
Abstract：In NH4Cl-NH3·H2O buffer solution of pH 10.4, diazo salt of p-sulfodiazoben-zene chloride reacts with rutin, morin, genistin, quercetin, hesperidin, baicalin and daidzin to form color azo-compounds. These azo-compounds have an absorption maximum at 430 nm except for baicalin azo-compound，which has an absorption maｘimｕm at 380 nm. The sensitivity of azoic color reaction is reductive in the order of above flavonoids and the molar absorptivity is maximum for rｕtin and is minimal for daidｚin among these azo-compounds，i.e. 3.28×104 and 8.3×102 L·mol-1·cm-1 respectively. The proposed spectrophotometry has been applied to the determination of flavonoids in sophora seed and soybean cake. The RSDs of the method were found to be in the range of 0.75%-4.48%, and the recoveries were in the range of 99.3%-105.0%.
Key words：Flavonoid;Azoic color reaction;Spectrophotometry;Sophora seed;Soybean cake
余 倩1，陈小康2，罗宗铭1，杨 迟1，麦桦坤1 . 黄酮化合物的偶氮显色反应及其应用研究 [J]. 光谱学与光谱分析, 2004, 24(08): 991-994.
YU Qian1, CHEN Xiao-kang2, LUO Zong-ming1, YANG Chi1, MAI Hua-kun1 . Azoic Color Reaction of Flavonoids and Their Analytical Application . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(08): 991-994.
