Determination of Five Trace Elements(Pb, Cd, Se, As and Hg) in Human Whole Blood by Temperature-Controllable Wet Digestion and ICP-MS Technique
ZHANG Xiu-wu1, 2, LI Yong-hua1*, YANG Lin-sheng1, LI Hai-rong1, WANG Wu-yi1
1. Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China
摘要： 采用温控HNO3-H2O2湿法消解，ICP-MS测定全血中Pb, Cd, Se, As, Hg 5种微量元素的含量。在选定的测试条件下，仪器系统稳定性良好，Pb等5种元素的检出限范围2～40 ng·L-1，全过程中样品均重复测定3次，RSD均在3%内。采用国家全血溶液标准物质GBW(E)09034-09036和人发标准物质GBW09101b中的相应元素进行质量控制，检测结果和推荐值相吻合。同时，应用本方法实测了湘西汞矿区34名村民全血中的5种元素的含量，结果表明：矿区村民Se和As处于正常水平，血Pb、血Cd、血Hg含量均偏高，有较高的健康风险，应当引起有关部门的重视。本方法快速、灵敏，精密度及准确度均符合要求，适于人体全血样中Pb, Cd, Se, As, Hg等微量元素的测定。
关键词：电感耦合等离子体质谱法;微量元素;重金属;全血;湿法消解
Abstract：A method for the determination of five trace elements, including Pb, Cd, Se, As and Hg concentrations in human whole blood by HNO3-H2O2 digestion under temperature-controllable condition and ICP-MS was described. Under the optimum experimental conditions and instrumental operation parameters, the limit of detection for the five elements were 2-40 ng·L-1. The system had a good stability with RSDs of the determination of value repeated 3 times less than 3%. The accuracy of the method was evaluated by the results of determination of the Chinese national standard references GBW(E)09034-09036 and GBW09101b, and the analytical results well agreed with the certified values. Using this method, the concentration of the five elements in whole blood of 34 habitants from mercury mining area in western Human province was studied. The result showed that the blood Se and blood As level was at security scope, however, blood Pb, Cd and Hg level was too high and beyond the safety thresholds which should be concerned about. The analytical method mentioned above can be used for determination of trace elements in human blood to achieve a convenient and rapid operation and accurate result.
张秀武1, 2, 李永华1*, 杨林生1, 李海蓉1, 王五一1 . 温控湿法消解ICP-MS测定全血中铅镉硒砷汞5种微量元素 [J]. 光谱学与光谱分析, 2010, 30(07): 1972-1974.
ZHANG Xiu-wu1, 2, LI Yong-hua1*, YANG Lin-sheng1, LI Hai-rong1, WANG Wu-yi1 . Determination of Five Trace Elements(Pb, Cd, Se, As and Hg) in Human Whole Blood by Temperature-Controllable Wet Digestion and ICP-MS Technique . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(07): 1972-1974.
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