摘要： 采用火焰原子吸收光度法分别测定了对数初、中、末期无菌与带菌盐藻细胞及上清液中Mg，Fe，K，Ca，Na，Mn，Zn，Cu，Ni，Cd和Cr的含量。结果表明：(1)该盐藻Mg，Fe，K，Ca和Na的含量在1～10 mg·g-1之间，Zn，Cu，Mn和Ni的含量基本在0.1～1 mg·g-1之间，有害金属Cd和Cr的含量极微。(2)无菌与带菌盐藻不同时期矿质元素的变化趋势大致相同，Mg, Fe, K, Ca, Na, Mn和Cu的含量随着培养时间的延长有所下降，Ca下降的最明显;Mg, Fe, K, Ca和Na的含量在上清液中保持稳定，无明显差异，而Cu和Mn含量在对数中后期的上清液中明显增加。(3)无菌与带菌盐藻Mg, K, Cu和Ni的含量差异不大;带菌盐藻的Fe, Ca, Na和Zn的含量在对数中期均显著下降，且低于无菌盐藻;而到对数末期又明显高于无菌盐藻。本实验结果为进一步利用盐藻资源以及深入研究藻菌相互作用机理提供了参考。
关键词：矿质元素;盐藻;原子吸收分光光度计;无菌
Abstract：The contents of eleven mineral elements, including Mg, Fe, K, Ca, Na, Mn, Zn, Cu, Ni, Cd and Cr contents of axenic and natural Dunaliella salina and their culture supernatants in the different period of exponential phase were determined with flame atomic absorption spectrophotometer(AAS). The results show as follows : (1)The contents of Mg, Fe, K, Ca, and Na are between 1 and 10 mg·g-1. The contents of Zn, Cu, Mn, and Ni are between 0.1 and 1 mg·g-1. There are little Cd and Cr in the microalgae. (2) The changes in the content of mineral elements of axenic and natural Dunaliella salina during different phases are almost the same. The contents of Mg, Fe, K, Ca, Na, Mn and Cu decreased along with the growth of the microalgae, especially the content of Ca. The contents of Mg, Fe, K, Ca and Na in the culture supernatants keep stable in the culture process and have no distinct difference among axenic and natural Dunaliella salina. But the contents of Cu and Mn in the culture supernatants increased greatly in the middle and end of exponential phase. (3) The contents of Mg, K, Cu, and Ni show no significant differences in axenic and natural microalgae. The contents of Fe, Ca, Na and Zn in the natural microalgae decreased greatly in the middle of exponential phase and were less than in axenic one, but increased at the end of exponential phase and were higher than in axenic one. These results provide reference for further to applying the resource of Dunaliella salina and studing the relationship of microalgae and associated bacteria in the culture.
唐 颖1，王长海2*，黄 笛1 . 无菌与带菌盐藻矿质元素含量的比较分析[J]. 光谱学与光谱分析, 2010, 30(07): 1956-1959.
TANG Ying1, WANG Chang-hai2*, HUANG Di1 . Analysis of the Mineral Element Contents of Axenic and Natural Dunaliella Salina. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(07): 1956-1959.
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