Application of Molecular Absorption Spectrophotometric Method to the Determination of Biologic Macromolecular Structures
CAO Shu-xia1,ZHAO Yu-fen1, 2*
1. Department of Chemistry, Zhengzhou University, Zhengzhou 450052,China 2. Department of Chemistry, the Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China
Abstract:A review on recent applications of molecular absorption spectrophotometric method to the identification of the structures of biologic macromolecules, such as protein and nucleic acid, is presented. Molecular absorption spectrophotometric method is widely used in the structure determination of biologic macromolecules for its convenience and speed. Ultraviolet absorption spectrum (UV) can be used in the research on the mechanism of the interaction of anticancer drugs and DNA. UV can also be used to study the interaction of spectroscopic probe with biologic molecule and their binding mechanism. Protein secondary structure and conformation can be investigated by Fourier infrared spectroscopy (FTIR) deconvolution analysis. Molecular absorption spectrophotometric method is an important tool for structure study of biologic macromolecules.
[1] Chalasinski G, Szczesniak M M. Chem. Rev., 1994, 94(7): 1729. [2] Zhang H P, Zhang R F, Shen J C. Supramolecular Science, 1998, (5): 627. [3] Conslable E C, Wand M D. J. Am. Chem. Soc., 1990, 112(3): 1256. [4] WANG Ya-li,WANG Hai-fang(王亚俐,王海芳). Acta Scientiarum Naturalium Universitatis Pekinensis(北京大学学报),2002, 38(2):159. [5] ZHU Bing,DU Xiu-lian,LI Rong-chang,WANG Kui(朱 兵, 杜秀莲, 李荣昌,王 夔). Chem. J. Chinese Universities(高等学校化学学报),2001,22(1):26. [6] ZHOU Yan-jiao,LI Ling-yuan,RU Bing-gen(周妍娇,李令媛,茹炳根). Chin. J. Biochem. Mol. Biol.(中国生物化学与分子生物学报),2000,16(4):483. [7] WEI Xiao-fang,DING Xi-ming,LIU Hui-zhou(魏晓芳,丁西明,刘会洲). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2000,20(4):556. [8] HU Qiu-luan,LI Ke-an(胡秋娈,李克安). Acta Scientiarum Naturalium Universitatis Pekinensis(北京大学学报),1999,35(6):850. [9] CHI Yan-hua,ZHUANG Jia,YI Xian-hua et al(迟燕华,庄 稼,易显华等). Chin. J. Appl. Chem.(应用化学),2000,17(1):26. [10] LI Na,LI Ke-an,TONG Shen-yang(李 娜,李克安,童沈阳). J. Anal. Sci.(分析科学学报),1997,13(4):265. [11] WEI Yong-ju,LI Ke-an,TONG Shen-yang(魏永巨,李克安,童沈阳). Chem. J. Chinese Universities(高等学校化学学报),1996,17(4):550. [12] WANG Hai-ren,XIAO Zhong-bai,SONG Gong-wu,ZHANG Hong-ju(王海人,肖忠柏,宋功武,詹红菊). Chinese J. Instr. Anal.(分析测试学报),2001, 20(4):45. [13] CHI Yan-hua,ZHUANG Jia,DONG Fa-qin et al(迟燕华,庄 稼,董发勤等) . Chinese J. Instr. Anal.(分析测试学报),1999,18(1):9. [14] WU Hui-ling,LI Wen-you,HE Xi-wen et al(吴会灵,李文友,何锡文等). Chin. J. Appl. Chem.(应用化学),2002,19(7):672. [15] CHI Yan-hua,ZHUANG Jia,LI Ke-an et al(迟燕华,庄 稼,李克安等) . Chinese J. Instr. Anal.(分析测试学报),2002,21(1):15. [16] HU Qiu-luan(胡秋娈). Chinese Chemical World(化学世界),1999,5:263. [17] LUO Zong-ming,ZHANG Kun,CUI Ying-de, DONG Fei-yan(罗宗明,张 琨,崔英德,董飞燕). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2001,21(2):251. [18] CHEN Yong,LI Yuan-zong,CHANG Wen-bao, CI Yun-xiang(陈 勇,李元宗,常文保,慈云祥). Chin. J. Anal. Sci.(分析科学学报),1994,10:67. [19] Kumar C V, Asuncion E H. J. Am. Chem. Soc., 1993, 115: 8574. [20] James B R, Meng G G, Posakony J J et al. Metal-based Drugs, 1996, 3: 85. [21] LIU Jie,XU Dong-hui,JI Liang-nian et al(刘 杰,许东晖,计亮年等). Chem. J. Chinese Universities(高等学校化学学报),2001,22(9):1446. [22] HUANG Cheng-zhi,LI Ke-an,TONG Shen-yang(黄承志,李克安,童沈阳). Chem. J. Chinese Universities(高等学校化学学报),1997,18(4):525. [23] ZHANG Zhi-gang,YANG Pin(张志刚,杨 频). Chem. J. Chinese Universities(高等学校化学学报),1997,18(8):1259. [24] LU Ji-xin,ZHANG Gui-zhu,WANG Yue-mei(卢继新,张贵珠,王月梅). Chinese J. Anal. Chem.(分析化学),2001,29(2):192. [25] LU Ji-xin,ZHANG Gui-zhu,HUANG Zhi-na,ZHAO Peng(卢继新,张贵珠,黄志娜,赵 鹏). Acta Chimica Sinica(化学学报),2002,60(6):967. [26] WANG Zi-chun,HUANG Deng-yu,HAO Jun-shan, YUAN Jing-ming(王自春,黄登宇,郝军山,袁静明). Chinese Journal of Antibiotics(中国抗生素杂志),1994,19(6):464. [27] ZHOU Jia-hong,JIANG Hui-jun,FENG Yu-ying et al(周家宏,姜慧君,冯玉英等). Chin. J. Appl. Chem.(应用化学),2001,18(12):994. [28] CAO Ying,HE Xi-wen(曹 瑛,何锡文). Chem. J. Chinese Universities (高等学校化学学报),1998,19(5):714. [29] LIU Dian-jun,WANG Zhen-xin,DONG Shao-jun(刘殿骏,王振新,董绍俊). Chinese Journal of Analysis Laboratory(分析试验室),2002, 21(4):54. [30] Ye Yong, Hu Jiming, He Lian et al. Vibrational Spectroscopy, 1999, 20(1):1. [31] YE Yong,HU Ji-ming,ZENG Yun-e(叶 勇,胡继明,曾云鹗). Chinese J. Anal. Chem.(分析化学),2001,29(7):866. [32] WU Ming-hu,SONG Gong-wu,LING Lian-sheng, HE Zhi-ke, ZENG Yun-e(吴鸣虎,宋功武,凌连生,何治柯,曾云鹗). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2000,20(4):575. [33] SONG Gong-wu,FANG Guang-rong,FENG Jian(宋功武,方光荣,冯 健). Chinese J. Anal. Chem.(分析化学),2000,28(1):128. [34] Kauppinen J K, Moffatt D J, Mantsch H H, Cameron O G. Anal. Chem., 1983, 53: 1454. [35] Surewicz W K, Mantsch H H. Biochimica et Biophysica Acta, 1988, 952: 115. [36] Byler D M, Brouillette J N, Susi H. Spectroscopy, 1986, 1(3): 29. [37] Surewicz W K, Moscarello M A, Mantsch H H. J. Biochem., 1987, 262(18): 8598. [38] LI Xao-feng,XU Yi-zhuang,ZHANG Ting-fang, WU Jin-guang, XU Guang-xian(李晓峰,徐怡庄,张庭芳,吴瑾光,徐光宪). Spectroscopy and Spectral Analysis(光谱学与光谱分析),1996,16(2):24. [39] Byler D M, Susi H. Biopolymers, 1986, 25: 469. [40] Yamamoto T, Tasumi M. Canadian Journal of Spectroscopy, 1988, 33(5): 133. [41] Purcell J M, Susi H. J. Biochem. Biopys. Meth., 1984, 9: 193. [42] FU Xiao-hong,LIN Qi-shui(傅晓红,林其谁). Acta Biochimica et Biophysica Sinica(生物化学与生物物理学报),1997,29(2):213. [43] TONG Yi-ping,LI Wei,LIN Yan-wen(童义平,李 伟,林燕文). Spectroscopy and Spectral Analysis(光谱学与光谱分析),1999,19(5):704. [44] ZHOU Rui-ming,SHEN Yong-jia(周瑞明,沈永嘉). Journal of East China University of Science and Technology(华东理工大学学报),1997,23(4):423. [45] Theophanides T. Appl. Spectrosc., 1981, 35:461.