Determination of Selenium in Rat′s Organs by Graphite Microwave Dissolution
WANG Li-xin1, 3，HU Xiao-rong2，3，LI Hui3*
1. Institute of Chemical Materials, Chinese Academy of Engineering Physics, Mianyang 621900, China 2. College of Material Science and Bioengineering, Chengdu University of Technology, Chengdu 610059, China 3. College of Chemical Engineering, Sichuan University, Chengdu 610065, China
Abstract：The content of selenium in rat′s ten organs was determined by graphite furnace atomic absorption spectrometry with microwave dissolution. Inorganic palladium modifier was added into the sample to solve the problems of selenium volatilization and matrix disturbance as selenium in the sample is liable to receive serious loss during the course of digestion and cineration. The optimistic determination conditions were shown as follow, digestion reagent of 4 mL (10∶3)HNO3/H2O2, matrix modification of 50 μg·mL-1 palladium chloride, cineration temperature of 1 200 ℃ and atomization temperature of 1 800 ℃. Under such optimum conditions, the linear range is 0-80 ng·mL-1 and the detection limit is 1.83 ng·mL-1. The RSD is less than 8% and the average recovery rate is 97.6%. The method was shown to be precise, reliable, convenient and quick in selenium determination of various organism organs.
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