Prediction of NDF and ADF Concentrations with Near Infrared Reflectance Spectroscopy (NIRS)
BAI Qi-lin1, 4, CHEN Shao-jiang1*, DONG Xiao-ling2, MENG Qing-xiang2, YAN Yan-lu3, DAI Jing-rui1
1. National Maize Improvement Center of China, China Agricultural University, Beijing 100094，China 2. College of Animal Science and Technology, China Agricultural University, Beijing 100094，China 3. College of Information and Electrical Engineering, China Agricultural University, Beijing 100094，China 4. Crop Genetics Research Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 033031，China
Abstract：The NDF (Neutral Detergent Fiber) and ADF (Acid Detergent Fiber) concentrations in maize stalk were analyzed with 147 samples selected from 600 samples of different eco-environments, hybrids and inbred lines, development stages, and various parts of the plants in two years. The technique of near infrared reflectance spectroscopy (NIRS) and partial least square (PLS) regression were used to establish the models. The results showed that the calibration models developed by the spectral data pretreatment of the first derivative+vector normalization, and the first derivative+multivariate scattering correction were the best for NDF and ADF with the same spectral regions (7 501.7-5 449.8 cm-1 and 4 601.3-4 246.5 cm-1). All these models yielded coefficients of determination of calibration (R2cal) for NDF and ADF that are higher than 0.94, while R2cv and R2val ranged from 0.92 to 0.96 for cross and external validation. The root mean square error of estimation, root mean square error of cross validation, and root mean square error of prediction (RMSEE，RMSECV and RMSEP) for NDF and ADF ranged from 1.49 % to 1.81%. The models can be used to measure various samples in screening and evaluating quality constituents of silage maize.
白琪林1，4，陈绍江1*，董晓玲2，孟庆祥2，严衍禄3，戴景瑞1 . 近红外漫反射光谱法测定玉米秸秆NDF与ADF含量[J]. 光谱学与光谱分析, 2004, 24(11): 1345-1347.
BAI Qi-lin1, 4, CHEN Shao-jiang1*, DONG Xiao-ling2, MENG Qing-xiang2, YAN Yan-lu3, DAI Jing-rui1 . Prediction of NDF and ADF Concentrations with Near Infrared Reflectance Spectroscopy (NIRS). SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(11): 1345-1347.
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