1. Key Laboratory for Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China 2. Department of Physics, Liaocheng University, Liaocheng 252059, China
Abstract：Raman spectra of insulin solvents are presented before and after being exposed to the pulsed electric field with extremely low frequency 50 Hz(ELF). The covalences of the molecule were not affected and the changes of some secondary bonds such as hydrogen bonds and salt bonds were observed. Detailed analysis of these spectra indicates that the α-helix of insulin molecule was destroyed after the exposure, which is proved by the shift of the peak of the amide Ⅰ region toward higher wave number and by the appearance of several new peakss: 1 561 and 1 594 cm-1 . Uhe disulfides were affected by the weaken α-helix, and their vibrational moees were changed. Meanwhile the hydrogen bonding between the dimer are broken down which leads to the increase in the peak intensities at 1 002 and at 1 602 cm-1.
Key words：Insulin solvent;50 Hz pulsed electric field;Raman;Conformation changes
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