杜仲中活性成分分析条件的优化研究

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摘要：采用均匀设计法对对二甲氨基苯甲醛显色法测定桃叶珊瑚甙的显色条件进行了优化。建立了测定桃叶珊瑚甙的高灵敏方法(ε=4.3×10⁴ L·mol^-1·cm^-1)，线性范围为4.5～45 μg·mL^-1，其灵敏度较文献报道提高2.5倍。研究了显色剂用量、显色时间、显色温度、酸用量等因素对桃叶珊瑚甙显色反应的影响。该法应用于杜仲提取物中桃叶珊瑚甙的测定，结果令人满意。

关键词：桃叶珊瑚甙;对二甲氨基苯甲醛;分光光度法;均匀设计法

Abstract：The absorption spectroscopic determination of aucubin by condensation with p-dimethylaminobenzaldehyde was optimized in this paper. A high sensitive method was established and the linearity range of the method was from 4.5 to 45 μg·mL^-1. The influences of the factors, such as the dosage of coloring agent, reaction time, heating temperature and the dose of acid etc., on the system were studied. And the result indicated that all of the factors had obvious effects on the system. The method has been applied to determining the content of aucubin in the extract of Eucommia olive with satisfactory results.

Key words：Aucubin;p-dimethylaminobenzaldehyde;Absorption spectroscopy;Uniform experimental design

收稿日期: 2003-09-06 修订日期: 2003-12-26

中图分类号:

O657.3

通讯作者: 周春山

引用本文:

彭密军^1，2，周春山^2*，刘建兰¹，张敏¹，雷启福² . 杜仲中活性成分分析条件的优化研究[J]. 光谱学与光谱分析, 2004, 24(12): 1655-1658.
PENG Mi-jun^1,2，ZHOU Chun-shan^2*，LIU Jian-lan¹，ZHANG Min¹，LEI Qi-fu² . Study on Optimizing the Analysis Condition for Active Compound in Eucommia Olive . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1655-1658.

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https://www.gpxygpfx.com/CN/Y2004/V24/I12/1655