Abstract：The applications of the high sensitivity of Surface-Enhanced Raman Spectroscopy (SERS) in labeled immunoassay have proved to be very useful. In the sandwich structure of “immobile antibody-antigen-labeled antibody”, the authors prepareｄ the labeled antibody by attachｉｎｇ gold nanoparticles，which ｗａs first labeled with moleculｅ that haｄ SERS signal, to the antibody. In this article, colloids were labeled with Raman reporter thiophenol through S—Au bond on gold and formed surface-enhanced Raman scattering (SERS) reporter-labeled gold colloids. Then, goat anti-rat antibody ｗａｓ adsorbed onto the reporter-labeled gold colloids and formed reporter-labeled immunogold colloids. This paper studied factors ａｆｆｅｃｔｉｎｇ FＴ-SEＲS ｒｅｐｏｒｔｅｒ-ｌａｂｅｌｅｄ ｉｍｍｕｎｏｇｏｌｄ colloids such as the sizes of gold particles, the amount of thiophenol in gold colloids and processing time, and whether goat anti-rat antibody would influence the SERS of reporter-labeled gold colloids.
[1] QIU Li-qun，GU Ren-ao(仇立群，顾仁敖). Spectroscopy and Spectral Analysis (光谱学与光谱分析)，待出版. [2] WANG Zi-zheng(王自正). Modern Medical Labeled Immology(现代医学标记免疫学). Beijing: People′s Medical Military Press(北京: 人民军医出版社), 2000. [3] Rohr Ｔ E, Cotton Ｔ, Fan N, Tarcha P J. Anal. Biochem., 1989, 182：388. [4] Dou X, Takama Ｔ, Yamaguchi Y, Yamamoto H. Anal. Chem., 1997, 69：1492. [5] Robert J Ni，Lipert J, Dawson G B, Porter M D. Anal. Chem., 1999, 71：4903. [6] ZHU Zi-hua et al(朱梓华等). Chinese Journal of Ｐhysical Chemistry (物理化学学报), 1999, 15(11): 966. [7] Carron Keith T, Hurley L Gayle. J. Phy. Chem., 1991, 95(24): 9979. [8] HU Rui-sheng et al(胡瑞省等). Chinese Journal of Ｐhysical Chemistry(物理化学学报), 1999, 15(11): 961. [9] Joo H J，Kim M S，Kim K. J. Raman Spectrosc., 1987, 18：57. [10] Zeman E J, Schatyz C C. J. Phys. Chem., 1978, 91：634. [11] Handley G D A. Colloidal Gold : Principle, Methods and Applications, Edited by Hayat M A. San Diego: Academic Press，1989. 13.
