Abstract：A fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the determination of sulfamera (SMR) was developed. The fluorescein-labeled SMR and sulfamethazine (SMZ) were synthesized and purified by thin layer chromatography (TLC). The optimized SMR FPIA had a dynamic range from 5.4 to 218.8 ng·mL-1 with IC50 value of 23.4 ng·mL-1 and a detection limit of 2.3 ng·mL-1. The specificity of the FPIA for SMR was investigated using other 16 sulfonamides and the cross-reactivity for SMR, SMZ and sulfadiazine (SDZ) was 100%, 25% and 8.6%, respectively.
王战辉,张素霞,沈建忠*. 荧光偏振免疫分析测定磺胺甲基嘧啶研究[J]. 光谱学与光谱分析, 2008, 28(07): 1621-1625.
WANG Zhan-hui,ZHANG Su-xia,SHEN Jian-zhong*. Development of a Fluorescence Polarization Immunoassay for Sulfamerazine. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(07): 1621-1625.
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