FTIR and 2D-IR Spectroscopic Studies on Different Sources of Herbra Cistanche
XU Rong1, SUN Su-qin2, LIU You-gang3, CHEN Jun2*, LIU Tong-ning4, CHEN Shi-lin1
1. The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, National Engineering Laboratory for Breedng of Endangered Medicinal Materials, Beijing 100193, China 2. Department of Chemistry, Tsinghua University, Beijing 100084, China 3. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 4. Yongning Plantation of Hebra Cistanche, Yinchuan 750010, China
Abstract：In the present paper, Fourier transform infrared spectroscopy (FTIR) associated with second derivative infrared spectroscopy and two-dimensional correlation spectroscopy (2D-IR) were used to analyze the 3 different sources of Hebra cistanche. The results showed that these IR spectra showed their different macro-fingerprint features: the charactersistic peaks of C. deserticola were located at ~1 730, 1 633, 1 156, 1 081, 1 025 and 931 cm-1 and those of C. tubulosa were at 1 692, 1 631, 1 604, 1 516, 1 265 and 1 023 cm-1, respectively. The only two peaks at ~1 151 and 1 085 cm-1 of C. salsa were different from that of C. deserticola. As a result, the IR spectrum of C. deserticola could be identified obviously from that of the C. tubulosa, whose similar index was only 0.623 3, but it was very similar to that of C. salsa, whose similar index was up to 0.904 8, demonstrating very similar ingredients. However, the difference between C. deserticola and C. salsa was obvious in the second derivative IR spectra: the shape of the two peaks of C. deserticola at ~1 730 and 816 cm-1 were much sharper. In addition, the fingerprint characters in 2D-IR spectra were more visualized. The three kinds of herbs were quite different from each other in the number and intensity of autopeaks. Therefore, FTIR macro-fingerprint method can identify different sources of Hebra cistance fast, nondestructively and effectively.
[1] TU Peng-fei, HE Yan-ping, LOU Zhi-cen(屠鹏飞，何燕萍，娄之岑). China Journal of Chinese Materia Medica (中国中药杂志), 1994, 19(1): 3. [2] Pharmacopoeia Commission of Ministry of Health, the People’s Republic of China(中华人民共和国卫生部药典委员会编). Pharmacopoeia of the People’s Republic of China (中华人民共和国药典). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2000. 103. [3] The Endangered Species Import & Export Management Office of the People’s Republic of China, Endangered Species Scientific Commission PRC(中华人民共和国濒危物种进出口管理办公室, 中华人民共和国濒危物种科学委员会). Convention on International Trade in Endangered Species(CITES)(濒危野生动植物种国际贸易公约): Appendix Ⅱ, 2003. 41. [4] Pharmacopoeia Commission of Ministry of Health, the People’s Republic of China(中华人民共和国卫生部药典委员会编). Pharmacopoeia of the People’s Republic of China(中华人民共和国药典). Beijing: Chemical Industry Press(北京: 化学工业出版社), 2005. 90. [5] LI Jun-song，YAO Zhong-qing，YU Ming-xia(李俊松，姚仲青，俞明霞). Lishizhen Medicine and Materia Medica Reasearch(时珍国医国药), 2000, 11(4): 34. [6] ZHANG Mian，FU Xiao-li，WANG Sheng-yong(张 勉，付晓丽，王胜勇). Journal of Chinese Medical Materials(中药材), 2004, 27(6): 401. [7] CHEN Pei，GUO Yu-hai，WANG Bao-min，et al(陈 培，郭玉海，王保民，等). Chinese Traditional and Herbal Drugs(中草药), 2006, 37(9): 1399. [8] SUN Su-qin, LIU Jun, ZHOU Qun(孙素琴，刘 军，周 群). Chinese J. Anal. Chem.(分析化学), 2002, 30(2): 140. [9] LIU Gang, TAN Li-ping, DONG Qin(刘 刚，谭丽萍，董 勤). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(1): 80. [10] Noda I. Appl. Spectrosc.，2000，54: 994. [11] SUN Su-qin, ZHOU Qun, QIN Zhu(孙素琴，周 群，秦 竹). Atlas of Two-Dimensional Correlation Infrared Spectroscopy for Traditional Chinese Medicine Identification(中药二维相关红外光谱鉴定图集). Beijing: Chemical Industry Press(北京：化学工业出版社), 2003. 1, 193. [12] XIE Jing-xing, CHANG Jun-biao, WANG Xu-ming(谢晶曦，常俊标，王绪明). Applications of IR Spectra in Organic Chemistry and Pharmaceutical Chemistry(红外光谱在有机化学和药物化学中的应用). Beijing: Science Press(北京：科学出版社), 2001.
