Abstract：For the need of biochemical chip, which consumes fewer specimens and is easy to integrate with micro-fluid chip, two kinds of spectrophotometric analysis methods are described in the present paper. Both the direct detection method and evanescent wave detection method are used in the experiments with visible light(460-800 nm). The experimental results proved that the direct detection is simple and evident; on the other hand the evanescent wave detection method consumes much less reagent and is easy to integrat with microchips.
[1] Splawn Bryan G, Lytle Fred E. Anal. Bioanal. Chem., 2002, 373: 519. [2] Mendes Sergio B, Li Lifeng, Burke James J. Langmuir，1996, 12: 3374. [3] Kumar P Suresh, Vallabhan C P G. Journal of Optics, A: Pure And Applied Optics，2002，4：247. [4] JIN Wei，LIAO Yan-biao，ZHANG Zhi-peng(靳 伟，廖延彪，张志鹏). Wave-guide Sensors: Principle and Technology(导波光学传感器：原理与技术). Beijing: Science Press(北京：科学出版社)，1998. 263. [5] GUO Shuo-hong(郭硕鸿). Dynamoelectric Mechanics(电动力学). Beijing: Higher Education Press(北京：高等教育出版社)，1995. 132. [6] GUO Fen-tai-xiong(国分太雄). Translated by Wang Yougong(王友功) . Lightwave Project(光波工程). Beijing：Science Press(北京：科学出版社)，2002. 31，48，131，165. [7] Haddock Hong S，Shankar P M. Sensors and Actuators，2003, B88: 67. [8] YU Fan, HUANG Shou-jiang，GONG Guang-lin, ZENG Zhao-quan, LI Ma-lin(俞 帆，黄守江，龚光林，曾昭权，李玛琳). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2001，21(2)：227.
