Abstract:The article puts milk forward as simulate sample for blood glucose measurement. With the new simulate sample, the authors make deep researches on measuring method and wavelength choice in the field of noninvasive blood glucose measurement by near-infrared diffuse reflectance spectroscopy. Depending on the absorption coefficient of lactose, the authors choose the two wavebands from 1 560 to 1 750 nm and form 2 090 to 2 190 nm. The model was built by partial least-squares(PLS), several pretreatment methods (including multiplicative scatter correction,first derivative and vector normalization) and different spectral regions were compared. The best correlation values for lactose were 0.99 and the best RMSEP values for lactose were 0.045. The results provide us with valuable theory reference and practical experiences and lead us to next research on noninvasive blood glucose measurement.
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