Determination of Inorganic Elements in the Soil-Grass-Animal System by Sealed Microwave Digestion ICP-AES
XIN Guo-sheng1,2, HU Zheng3, ZHOU Wei4, YANG Zhi-qiang1, GUO Xu-sheng2,5, LONG Rui-jun1,2,5*
1. College of Grassplot Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, China 2. The International Center for Tibetan Plateau Ecosystem Management, Lanzhou University, Lanzhou 730000, China 3. Testing & Analysis Center, Lanzhou University, Lanzhou 730000, China 4. Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730020, China 5. College of Life Science, Lauzhou University, Lanzhou 730000, China
Abstract:The contents of inorganic elements including K, Ca, Na, Mg, P, S, Fe, Cu, Mn, Zn, Mo, and Co in the soil-grass-animal mineral system from Qinghai Tibetan Plateau were determined by ICP-AES using high pressure system-sealed microwave digestion. The sample of soil was digested with HNO3-HF-H2O2 acids system, but others including pasture, animal fur, liver, and serum were digested with HNO3-H2O2 acids system. The operation would be simplified and the blank value would be decreased with the above acids systems. The results were proved to be reliable with the relative standard deviation being 0.271%-2.633% for Ca, 2.971%-4.854% for Co, 0.372%-2.874% for Cu, 0.600%-3.683% for Fe, 0.347%-2.829% for K, 0.626%-2.593% for Mg, 0.705%-4.828% for Mn, 2.946%-4.622% for Mo, 0.689%-3.621% for Na, 0.422%-3.890% for P, and 0.143%-4.622% for S, 0.166%-2.399% for Zn, and all of them were less than 5% for all the elements, and the recovery being 97.1%-101.4% for Ca, 93.5%-112.5% for Co, 95.2%-104.0% for Cu, 96.9%-104.2% for Fe, 96.1%-105.6% for K, 96.2%-102.8% for Mg, 91.5%-108.9% for Mn, 95.0%-113.5% for Mo, 95.2%-101.8% for Na, 94.7%-100.7% for P, 98.3%-108.4% for S, and 97.5%-102.0% for Zn by adding standard recovery experiment. The results of determination were proved that the method of sealed microwave digestion ICP-AES was sensitive, precise, easy to operate and rapid for the determination of inorganic elements in the soil-grass-animal mineral system, and could satisfy the sample examination request. The methods and results were meaningful to research on the soil-pasture-animal mineral system including the contents of mineral elements, the circulation of mineral elements, the interaction, and the application of mineral additive.
Key words:Qinghai Tibetan Plateau;Soil-grass-animal mineral system;ICP-AES;Macro element;Trace element
辛国省1,2,胡 征3 ,周 围4,杨志强1,郭旭升2,5,龙瑞军1,2,5* . 微波密闭消解ICP-AES法测定土-草-畜系统中无机元素[J]. 光谱学与光谱分析, 2010, 30(02): 546-550.
XIN Guo-sheng1,2, HU Zheng3, ZHOU Wei4, YANG Zhi-qiang1, GUO Xu-sheng2,5, LONG Rui-jun1,2,5* . Determination of Inorganic Elements in the Soil-Grass-Animal System by Sealed Microwave Digestion ICP-AES . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 546-550.
[1] Iain T. Applied Geochemistry, 2002, 17: 1017. [2] Abrahams P W. Journal of Chemical Ecology, 1999, 25: 2215. [3] HU Zi-zhi, WEN Fen-wu, LU Tai-an(胡自治, 文奋武, 卢泰安). Acta Prataculturae Sinica(草业学报), 1999, 8(2): 63. [4] Abreu M M, Matias M J, Magalhaes M C F, et al. Journal of Geochemical Exploration, 2008, 96: 161. [5] McDowell L R. Animal Feed Science Technology, 1996, 60: 274. [6] Long R J, Dong S K, Wei X H, et al. Livestock Production Science, 2005, 93: 197. [7] DONG Shi-kui, LONG Rui-jun, HU Zi-zhi(董世魁,龙瑞军,胡自治). Acta Prataculturae Sinica(草业学报), 2000, 9(2): 32. [8] Wilde D. Animal Reproduction Science, 2006, 96: 240. [9] Hostetler C E, Kincaid R L, Mirando M A. The Veterinary Journal, 2003, 166: 125. [10] Heaton K, Kelly S D, Hoogewerff J. Food Chemistry, 2008, 107: 506. [11] Ogebe P O, Ogunmodede B K, McDowell L R. Small Ruminant Research, 1996, 19: 193. [12] Kuss H M, Fresenius J. Anal Chem., 1992, 343: 788. [13] Binstock D A, Groohseetal P M. Asso. of Anal. Chem., 1991, 7(2): 6. [14] QIAN Hong-sen(钱鸿森). Microwave Heat up Technology and Application(微波加热技术及应用). Harbin: Heilongjiang Science Press(哈尔滨:黑龙江科学出版社),1985. [15] FENG Da-he, YU Bi-yu, CHENG Yu-long(冯大和,余碧钰,程玉龙). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 2001, 18(5): 483. [16] JIN Qin-han(金钦汉). Chinese Journal of Anal. Chem.(分析化学), 1998, 26(7): 668. [17] CHEN Su-lan, HU Guan-jiu, CHEN Bo, et al(陈素兰,胡冠九,陈 波,等). Arid Environmetal Monitoring(干旱环境监测), 2006, 20(2): 69. [18] YU Ke-li(宇克莉). Journal of Tianjin Normal University·Natural Science Edition(天津师范大学学报·自然科学版), 2007, 27(3): 6. [19] WANG Ji-sheng, WANG He-qun, ZHENG Yong-jun(王继生,王鹤群,郑永军). Journal of Jinan University(济南大学学报), 2008, 22(1): 59. [20] HOU Tian-ping, WANG Song-jun, CAO Lin, et al(侯天平,王松君,曹 林,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2008, 28(8): 1933. [21] HAN Chao, LIU Cui-ping, ZHAN Xiu-ming(韩 超,刘翠平,詹秀明). Journal of Analytical Science(分析科学学报), 2008, 24(1): 91.