Studies on the Determination of Protein with Spectroscopic Probe of o-Chlorophenylfluorone-Molybdenum(Ⅵ) Complex in Microemulsion Medium
DU Bin1, LI Yan1, ZHANG Hui1, WU Dan1, WEI Qin1, LI Zai-jun2
1.School of Chemistry and Chemical Engineering, Ji'nan University, Ji'nan 250022, China 2.College of Chemical and Material Engineering, Southern Yangtze University, Wuxi 214036, China
Abstract：The interaction and absorption spectral behavior of o-chlorophenylfluorone (o-CPF)-Mo(Ⅵ) and protein are studied in OP microemulsion medium.Under the optimum condition, the molar absorptivity of o-CPF-Mo(Ⅵ)-BSA system at 532 nm is 6.12×106 L·mol-1·cm-1, and Beer’s law is obeyed in the range of 0-14 μg·mL-1 of BSA.Moreover, the binding number between the complex and BSA determined by molar ratio and slope ratio methods is 91.The mechanism of interaction of BSA and o-CPF-Mo(Ⅵ) is also discussed.OP microemulsion media introduced into the protein determination increased markedly the sensitivity of the system.The result of experiment indicates that the method possesses a high sensitivity as well as a high selectivity.It can be used to determine protein in human urine with satisfactory results.
杜 斌1，李 燕1，张 慧1，吴 丹1，魏 琴1，李在均2 . 微乳液介质-配合物光谱探针高灵敏光度法测定微量蛋白质的研究[J]. 光谱学与光谱分析, 2005, 25(07): 1110-1113.
DU Bin1, LI Yan1, ZHANG Hui1, WU Dan1, WEI Qin1, LI Zai-jun2 . Studies on the Determination of Protein with Spectroscopic Probe of o-Chlorophenylfluorone-Molybdenum(Ⅵ) Complex in Microemulsion Medium. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1110-1113.
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