流动注射化学发光法测定环丙沙星

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摘要：环丙沙星(CPLX)在酸性介质中与Tb³⁺形成配合物，通过能量转移反应对Ce(Ⅳ)-SO^2-₃化学发光体系有很强的增敏作用。据此，采用流动注射技术，建立了流动注射稀土敏化化学发光法测定CPLX的新方法。在9.0×10^-9～1.0×10^-6 mol·L^-1范围内CPLX浓度与化学发光强度呈良好的线性关系，方法的检出限为2.2×10^-11 mol·L^-1，对5.0×10^-8 mol·L^-1 CPLX平行测定11次，相对标准偏差为3.0%。利用该法测定了胶囊和生物体液中CPLX的含量，方法简单快速，重现性好，结果令人满意。

关键词：流动注射;化学发光;铽;环丙沙星;胶囊;血清;尿样

Abstract：A new flow injection chemiluminescence (CL) method for the determination of Ciprofloxacin (CPLX) was described.The method was based on the weak CL reaction of Ce(Ⅳ)-SO^2-₃ sensitized by Tb³⁺-CPLX.The relative CL intensity was linear with the concentration of CPLX in the range of 9.0×10^-9-1.0×10^-6 mol·L^-1.The detection limit is 2.2×10^-11 mol·L^-1.The relative standard deviation (RSD) for the determination of 5.0×10^-8 mol·L^-1 CPLX is 3.0% (n=11).The method has been successfully applied to the analysis of CPLX in tablet and capsule.An original mechanism was proposed to explain this phenomenon.

Key words：Flow injection;Chemiluminescence;Terbium;Ciprofloxacin;Capsule;Serum;Urine

收稿日期: 2004-02-02 修订日期: 2004-06-09

中图分类号:

O657.3

通讯作者: 常希俊

引用本文:

连宁^1，2，王建朝²，冷文红³，常希俊^1* . 流动注射化学发光法测定环丙沙星[J]. 光谱学与光谱分析, 2005, 25(07): 1038-1041.
LIAN Ning^1，2,WANG Jian-chao²,LENG Wen-hong³, CHANG Xi-jun^1* . Flow Injection Analysis of Ciprofloxacin by Terbium Sensitized Chemiluminescence Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1038-1041.

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https://www.gpxygpfx.com/CN/Y2005/V25/I07/1038