Spectroscopy Research on the Origin Mechanism for Light Induced Delayed Fluorescence of Chloroplast
WANG Cheng-long1, MA Guo-xin1, 2，FAN Duo-wang1, XIA Rong-bin1
1.Key Laboratory of Opto-electronic Technology and Intelligent Control, Ministry of Education, Lanzhou Jiaotong University, Lanzhou 730070, China 2.College of Electronics and Information Engineering, South China University of Technology, Guangzhou 510640, China
Abstract：Research of the mechanism for delayed fluorescence origin play an important role in the application of DF.Based on the charge recombination theory, the mechanism for delayed fluorescence origin is theoretically analyzed in this paper.The decay kinetics of DF, depending on the backward transport electron and oxidized state P680+, can be well fitted by poly-exponent.The relative fast component of DF originates from the Z+P680 Q-A states, generated during the previous period of the sample illumination.On the other hand, the slow component of DF is ascribed to the state Q=B—the next electron carrier of deoxidized side in PSⅡ.Meanwhile, the experimental results confirmed the theoretical conclusion.Furthermore, the authors testified that the third component with lifetime much greater than the time of the registration period (which is presented as a constant), is corresponds to the recombination of P680+ and the backward transport electron from PSⅠ.
王成龙1，马国欣1，2，范多旺1，夏荣斌1 . 叶绿体光诱导延迟荧光产生机理的光谱学研究[J]. 光谱学与光谱分析, 2005, 25(08): 1262-1265.
WANG Cheng-long1, MA Guo-xin1, 2，FAN Duo-wang1, XIA Rong-bin1 . Spectroscopy Research on the Origin Mechanism for Light Induced Delayed Fluorescence of Chloroplast. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(08): 1262-1265.
[1] Strehler B, Arnold W.J.Gen.Physiol., 1951, 34: 809. [2] Itoh S, Murata N.Photochem.Photobiol., 1973, 18(3): 209. [3] Marek Godlewski, et al.J.Photochem.Photobiol.B: Biol, 1993, 21: 29. [4] Chenglong Wang, Da Xing, Qun Cheng.Biosensors and Bioelectronics, 2004, 20： 454. [5] Balasigamani Devaraj, et al.Optical and Magnetic Materials，1997, 20: 188. [6] Gerhardt V, Bodemer U.Archiv für Hydrobiologie Special Issues Advances in Limnology, 2000, 55: 101. [7] WANG Cheng-long, XING Da, ZENG Li-zhang(王成龙, 邢 达, 曾礼漳).Spectroscopy and Spectral Analysis(光谱学与光谱分析).In press. [8] Popp F A, et al.Experientia, 1988, 44(7): 543. [9] Scott R Q, et al.Appl.Phys., B, 1989, 48: 183. [10] YE Ji-yu, LI De-yao , SHEN Yun-gang.Acta Phytophysiologia Sinica, 1995, 21(1): 73.
