Detection of the Expression of Transgene in Rice Plant Based on Hyperspectral Remote Sensing Technique
LI Ru1，CHEN Jin-song1*，YUAN Ding-yang2，TANG Li2，LIN Hui1，TAN Yan-ning2，YUE Yue-min3，HE Hai-xia4
1.Institute of Space and Earth Information Science, the Chinese University of Hong Kong, Hong Kong, China 2.China National Hybrid Rice R&D Center/Hunan Hybrid Rice Research Center, Changsha 410125, China 3.Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China 4.Institute of Remote Sensing Applications, Chinese Academy of Sciences, Beijing 100101, China
Abstract：The present study aims to identify the expression of transgene in given rice plant samples in certain conditions.To avoid external noise caused by temperature change and water-loss, field spectrum was collected with ASD field spectrometer in natural state.The study calculated the mean spectrum of samples as main data set analyzed which were controlled by inner clustering coefficient to ensure data quality.By mean spectrum, the noise from random distinctions in few individual cultivators, which could not be expressed in the class stably, could be weakened even with filtering.With the help of parameters, such as red edge and green peak, this study gave qualitative spectral differences between transgenic samples and their parents.The results show that the transgenes in rice plant were expressed and influenced the samples.Moreover, it was found that the parameters of area are more suitable for describing the differences/changes of the samples, while PRI and SR-PRI are more sensitive to indicate them.Most of the above results could be found on the continuum-removal spectrum curve of samples.These conclusive results demonstrate that hyperspectral remote sensing technique has good prospects and application potential in transgene expression detection and monitoring, especially in plant breeding process.
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