Studies on Aclacinomycin Spectral Characters and the Interaction between Aclacinomycin and DNA by Spectroscopic Method
HU Min1,2, ZHANG Zhen-xi1*,SHEN Guo-li3, LIU Ya-li3
1. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Engineering, Xi’an Jiaotong University, Xi’an 710049, China 2. Department of Applied Chemistry, School of Science, Xi’an Jiaotong University, Xi′an 710049, China 3. State Key Laboratory for Chemo/Biosensing Technology and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
Abstract：The interaction of aclacinomycin(ACR) and DNA was investigated by fluorescence spectrum, and the characteristics of the fluorescence and absorption of aclacinomycin (ACR) were studied. The results indicate that there are two situations: in the case that the concentration ratio of ACR to DNA is small, the ACR is intercalated into the stacked base pairs of DNA; in the other case that the concentration ratio of ACR to DNA is great, the interaction between ACR and DNA is complex. The binding constant of the interaction between ACR and DNA, calculated by the fluorescence titration method, is 2.7 ×106 mol·L-1, and the binding site number is about 0.67 base pairs.
胡敏1,2,张镇西1*,沈国励3,刘娅莉3. 阿克拉霉素A与DNA作用的光谱学研究[J]. 光谱学与光谱分析, 2006, 26(09): 1668-1671.
HU Min1,2, ZHANG Zhen-xi1*,SHEN Guo-li3, LIU Ya-li3 . Studies on Aclacinomycin Spectral Characters and the Interaction between Aclacinomycin and DNA by Spectroscopic Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(09): 1668-1671.
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