Study on Interaction between Sulfonylurea Herbicides and Catalase by Fluorescence Spectroscopy
YE Fa-bing1, 2, DONG Yuan-yan1*, ZHOU Peng2, MO Xiao-man2, HU Xian-wen1
1. Department of Chemistry, College of Science, Huazhong Agriculture University, Wuhan 430070, China 2. Department of Chemistry, Huanggang Normal University, Huanggang 438000, China
Abstract：The binding of Sulfonylurea herbicides to catalase in aqueous solution was studied using fluorescence spectroscopy. It was shown that herbicides have a strong ability to quench the catalase fluorescence mainly through a static quenching procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. For chlorsufuron, K=8.69×105 L·mol-1 and n=1.16; for metsufuron methyl, K=1.01×106 L·mol-1 and n=1.21; and for bensufuron methyl, K=3.52×103 L·mol-1, n=0.77. It is clear that the binding of metsufuron methyl with catalase is stronger than that of chlorsufuron, while the binding of chlorsufuron stronger than that of bensufuron methyl.
叶发兵1, 2,董元彦1*, 周鹏2,莫小曼2,胡先文1 . 磺酰脲除草剂与过氧化氢酶的相互作用[J]. 光谱学与光谱分析, 2006, 26(09): 1685-1687.
YE Fa-bing1, 2, DONG Yuan-yan1*, ZHOU Peng2, MO Xiao-man2, HU Xian-wen1 . Study on Interaction between Sulfonylurea Herbicides and Catalase by Fluorescence Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(09): 1685-1687.
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