Colon Adenoma Detection Using Kubelka-Munk Spectral Function of DNA and Protein Bands
WEI Hua-jiang1,GUO Zhou-yi1*,XIE Shu-sen2,HE Bo-hua3,LI Li-bo3,CHEN Xue-mei4,WU Guo-yong5, LU Jian-jun5
1. Key Laboratory of Laser Life Science and Institute of Laser Life Science of Ministry of Education, South China Normal University, Guangzhou 510631, China 2. Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007, China 3. Department of Surgery, First Affiliated Hospital of Guangdong College of Pharmacy, Guangzhou 510224, China 4. Department of Ophthalmology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China 5. Department of Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China
Abstract：Differential diagnosis of human colon adenoma was studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 590 to 1 064 nm pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r∞) and logarithmic Kubelka-Munk function log[f(r∞)] of the DNA absorption bands at 260 nm between normal and adenomatous colon epithelial tissues, and the differences were 218% (p＜0.05) and 68.5% (p＜0.05) respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r∞) and logarithmic Kubelka-Munk function log[f(r∞)] of the protein absorption bands at 280 nm between normal and adenomatous colon epithelial tissues, and the differences were 208%(p＜0.05) and 59.0%(p＜0.05) respectively. Pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r∞) and logarithmic Kubelka-Munk function log[f(r∞)] of the β-carotene absorption bands at 480 nm between normal and adenomatous colon epithelial tissues, and the differences were 41.7%(p＜0.05) and 32.9%(p＜0.05) respectively. Obviously, pathological changes of colon epithelial tissues were induced so that there were significant changes in the contents of the DNA, protein and β-carotene of colon epithelial tissues. The conclusion can be applied to rapid, low-cost and noninvasive optical biopsy of colon adenoma, and provides a useful reference.
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