Identification of Cistanche Deserticola from Boschniakla Rossica and Cynomorium Songaricum Using FTIR and Two-Dimensional Correlation IR Spectroscopy
CHEN Jun1，2, SUN Su-qin3, XU Rong1*, LIU You-gang4, YU Jing1, LIU Tong-ning5, LI Jian-qiang2
1. The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China 2. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100194, China 3. Department of Chemistry, Tsinghua University, Beijing 100084, China 4. Tianjin University of Traditional Chinese Medicine，Tianjin 300193, China 5. Ningxia Yongning Plantation of Herba Cistanche, Yinchuan 750100, China
Abstract：In the present paper, Fourier transform infrared spectroscopy (FTIR) combined with two-dimensional correlation spectroscopy (2D-IR) was used for the identification of Cistanche deserticola from its adulterants, Boschniakla rossica and Cynomorium songaricum. The results showed that these IR spectra showed their different macro-fingerprint feathers: the charactersistic peaks for Cistanche deserticola were located at ～1 730 and 931 cm-1. While absorption peaks at ～1 510, 1 375 and 1 266 cm-1 were only found in Boschniakla rossica. And the strongest peak of Cynomorium songaricum was at 1 614 cm-1. Differences were more obvious in the second derivative IR spectra: the intensities of the four peaks of Cistanche deserticola at ～1 453, 1 336, 931, and 892 cm-1 were strong, among which the peak at ～931 cm-1 was sharp and the strongest in intensity. There was the strongest peak at ～1 509 cm-1 for Boschniakla rossica. In addition, the shape of peaks at ～1 633 and 1 161 cm-1 was wider and the intensities were stronger. While the shape of peak at ～1 682 cm-1 was sharp, and also the intensity of peak at 1 605 cm-1 was stronger. According to the features of the IR spectra of each kind, a quick and accurate method based on FTIR for the identification of Cistanche deserticola was established.
陈君1，2,孙素琴3,徐荣1*,刘友刚4,于晶1,刘同宁5, 李健强2 . 应用红外光谱法鉴别肉苁蓉及其混淆品草苁蓉和锁阳[J]. 光谱学与光谱分析, 2009, 29(06): 1502-1507.
CHEN Jun1，2, SUN Su-qin3, XU Rong1*, LIU You-gang4, YU Jing1, LIU Tong-ning5, LI Jian-qiang2 . Identification of Cistanche Deserticola from Boschniakla Rossica and Cynomorium Songaricum Using FTIR and Two-Dimensional Correlation IR Spectroscopy . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(06): 1502-1507.
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